Novel accelerated in vitro breeding for phytoremediation

用于植物修复的新型加速体外育种

• 批准号: 7288753
• 负责人: Mihaly Czako
• 金额: $ 31.18万
• 依托单位: SOUTHERN SUN BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7288753
• 关键词: Acceleration; Acclimatization; Biochemical; Biomass; Books; Breeding; Businesses; Cell division; Cellulose; Certification; Cities; Collaborations; Condition; Cultured Cells; Data; Dental Pulp; Drug Metabolic Detoxication; Environmental sludge; Family; Fermentation; Frequencies; Genetic Variation; Goals; Growth; Hydroponics; In Vitro; Intellectual Property; Letters; Licensing; Lignin; Liquid substance; Modification; Morphology; Numbers; Nurseries; Output; Performance; Phase; Physiological; Plants; Prevention; Process; Production; Protocols documentation; Range; Rate; Reporting; Resistance; Role; Sales; Sewage; Small Business Technology Transfer Research; Sodium Chloride; System; Technology; Technology Transfer; Testing; Transportation; Variant; commercialization; cost; dehalogenation; field study; improved; innovation; new technology; novel; pollutant; remediation; technology development; tissue culture; trait

DESCRIPTION (provided by applicant): The long-term goal of the collaboration between the USC and Southern Sun BioSystems is to create a plant breeding and remediation technology-development center which would supply hardware, protocols for breeding-selection and mass production of elite plants for nurseries and remediation businesses, with licensing and tracking mechanisms for protecting intellectual property. This project is built on the successful demonstration of the feasibility of our novel technology for in vitro breeding of a large biomass plant for field-scale phytoremediation. The technology transfer capitalizes on Southern Sun's innovative mass propagation technologies and on somaclonal breeding by amplification of the genetic variation-generating step, that is the number of cell divisions in the totipotent cell cultures subjected to selection. A commercialization plan for elite plant sales and breeding technology is supported by letters of commitment from a variety of remediation and biomass utilizing businesses. Phase II objectives: (1) the frequency and extent of somaclonal variation in second round of culture and (2) preselection using the same pollutant (testing physiological constraints on dehalogenation); (3) add valuable new traits (morphological variations, higher cellulose to lignin ratio) to elite lines from Phase I and (4) to a salt tolerant line; (5) improve mass propagation, transportation, acclimatization by prevention of hyperhydricity; (6) certify elite lines by determining first order detoxification rate constant in hydroponic system, (7) by testing stability of the new traits, and by crop trials to demonstrate remediation of trichlorophenol (and biomass production) of TCP at Southern Sun (8a), and to demonstrate biomass production and remediation of a select pollutant in city sewage sludge at BioTech, Inc., Cayce SC (8b), and potentially in hog lagoons in Clinton, NC. Phase II will produce: elite lines with certified superior haloorganic detoxification ability with added traits to promote end uses, sufficient in vitro stock for mass propagation/field applications, an improved strategic efficiency, cost per unit output, of elite plants for phytoremediation, and pre-booked sales.

描述（由申请人提供）：南加州大学和南方太阳生物系统公司之间合作的长期目标是建立一个植物育种和修复技术开发中心，该中心将为苗圃和修复业务提供硬件，育种选择和大规模生产优质植物的协议，并具有保护知识产权的许可和跟踪机制。这个项目是建立在我们的新技术的可行性的成功证明，在体外培育一个大型生物质植物进行田间规模的植物修复。这项技术转让利用了南方太阳创新的大规模繁殖技术和体细胞无性系育种，通过扩增遗传变异产生步骤，即在全能性细胞培养中进行选择的细胞分裂数量。优质植物销售和育种技术的商业化计划得到了各种修复和生物质利用企业的承诺书的支持。第二阶段目标：(1)第二轮培养中体细胞无性系变异的频率和程度；(2)使用相同污染物进行预选（测试脱卤的生理限制）；(3)在ⅰ期优良品系中添加有价值的新性状（形态变异、纤维素/木质素比提高）；(4)在耐盐品系中添加有价值的新性状；(5)通过防止过度缺水，提高大批量繁殖、运输和适应能力；(6)通过确定水培系统的一级解毒速率常数来认证优质品系；(7)通过测试新性状的稳定性，并通过作物试验来证明在Southern Sun （8a）对TCP的三氯酚（和生物质生产）进行修复；在BioTech， Inc., Cayce SC （8b）以及潜在的在Clinton， NC的猪泻湖进行生物质生产和对城市污水污泥中选定污染物的修复。二期将生产：经认证具有卓越的卤素有机解毒能力的优良品系，并增加性状以促进最终用途，有足够的体外库存用于大规模繁殖/现场应用，提高战略效率，提高用于植物修复的优良植株的单位产量成本，以及提前预订销售。