Mechanisms of Enhanced Resistance of E. coli Biofilms

大肠杆菌生物膜增强耐药性的机制

基本信息

批准号：
7246623
负责人：
MICHAEL R BENOIT
金额：
$ 4.6万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-01 至 2008-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7246623
关键词：
3-Dimensional Address Affect Animals Antibiotic Resistance Antibiotics Antimicrobial Resistance Architecture Bacteria Bacteria sigma factor KatF protein Biochemical Biological Process Bioreactors Brush Border Cell physiology Cells Characteristics Chronic Communicable Diseases Communities Condition DNA Microarray Chip DNA Microarray format Development Devices Disease Environment Escherichia coli Food Gene Expression Gene Expression Profile Gene Proteins Genes Genetic Transcription Genitourinary system Hour Human In Vitro Individual Infection Knock-out Knowledge Laboratories Laboratory Organism Liquid substance Location Mass Spectrum Analysis Mechanical Stress Mechanics Microbe Microbial Biofilms Microbiology Microgravity Modification Molecular Nutrient Operative Surgical Procedures Personal Satisfaction Phase Physiology Polysaccharides Process Proteins Proteome Research Resistance Resistance development Robin bird Role Route Safety Sigma Factor Simulate Space Flight Staging Stimulus Stress Surface System Texas Tissue-Specific Gene Expression Training Translations Two-Dimensional Gel Electrophoresis Virulence Virulent Water Supply Work bacterial resistance base cellular microvillus denitrification drinking water experience gastrointestinal gastrointestinal microvillus interest microbial multidisciplinary mutant novel prevent protein degradation protein protein interaction repository research study respiratory response shear stress skills tissue culture wasting water treatment yeast two hybrid system

项目摘要

DESCRIPTION (provided by applicant): Bacteria within a biofilm are more effective in phenomena that are detrimental to human well being. Based on the work of Dr. Matin and others, it is hypothesized that due to low-shear fluid effects, such as potentially altered macromolecular folding, and documented changes in gene expression, biofilms under low-shear conditions are different from their conventional counterparts. Using novel adaptations to a rotating wall vessel (RWV) bioreactor, devised in the Matin lab, E. coli biofilms will be cultivated in low-shear fluid environments. What unique genes and proteins are expressed at different developmental stages of the low-shear biofilms will be determined, using DNA microarrays, 2-D gel electrophoresis, and mass spectrometry. Mutants unable to form low-shear biofilms or unable to enhance resistance will be isolated. Use of knockouts in selected genes will further explore the role of individual genes in low-shear biofilm formation and antibiotic resistance. What effect RWVs may have on protein-protein interactions will be determined, using two hybrid and TAP-tagging systems, with suitable proteins. Fundamental and enabling information will result in better control of biofilms for preventing infectious disease.

描述（由申请人提供）：生物膜内的细菌在对人类健康有害的现象中更有效。基于Matin博士和其他人的工作，假设由于低剪切液效应（例如潜在改变的大分子折叠）以及基因表达的证明变化，低剪切条件下的生物膜与它们的常规对应物不同。使用对Matin Lab中设计的旋转壁血管（RWV）生物反应器的新型适应，将在低剪切液环境中培养大肠杆菌生物膜。使用DNA微阵列，2-D凝胶电泳和质谱法，将确定在低剪切生物膜的不同发育阶段表达的独特基因和蛋白质。无法形成低剪切生物膜或无法增强电阻的突变体被隔离。在选定基因中使用敲除将进一步探索单个基因在低剪切生物膜形成和抗生素耐药性中的作用。 RWV可能对使用合适的蛋白质的两个混合和TAP tagging系统确定RWV对蛋白质蛋白质相互作用的影响。基本和启示信息将更好地控制生物膜，以预防传染病。