Proteomic analysis of Mutator transposition

突变转座的蛋白质组学分析

• 批准号: 7188570
• 负责人: David S Skibbe
• 金额: $ 4.88万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7188570
• 关键词: Amino Acids; Behavior; Biochemical; Biological Assay; Candidate Disease Gene; Cell Proliferation; Complementary DNA; DNA; DNA Sequence; Databases; Development; Excision; Genes; Genome; Genomics; Histocompatibility Testing; Human; Integration Host Factors; Location; Maize; Mining; Molecular; Nature; Organism; Pattern; Process; Protein Isoforms; Proteins; Proteome; Proteomics; Role; Selection Criteria; Staging; Stretching; Tissues; Translations; Transposase; Viral Vector; base; gene therapy; genetic element; genome sequencing; in vivo; inhibitor/antagonist; positional cloning; prevent; repaired

DESCRIPTION (provided by applicant): Transposons are genetic elements that can move to new locations in the genome. They are ubiquitous in essentially all organisms, and recent genome sequencing projects in humans and maize have shown that transposons represent significant portions of both genomes. At the cellular level, transposon activity requires DNA and chromosomal repair processes to prevent cellular damage that could result in uncontrolled cell proliferation. Aside from these downstream studies on the consequences of transposon activity, relatively little is known about the proteins that modulate their upstream (excision and insertion) behavior in vivo. This study will use a proteomics-based approach to identify host proteins that regulate transposition behavior and potentially identify candidate proteins that modulate cell proliferation as well as establish a viable gene therapy alternative to viral vectors. The specific aims of this project are: (1) To compare the proteomes of tissues before and after activation of Mu; (2) to refine the spatial expression pattern of the transposition mechanisms; and (3) to mine genomic and cDNA databases to identify frame-shift translation candidate genes. The developmental role(s) of proteins associated with transposition in the four selected tissue types will be further analyzed using molecular, biochemical and reverse genetics approaches.

描述（由申请人提供）：转座子是可以移动到基因组中新位置的遗传元件。它们基本上普遍存在于所有生物体中，最近的人类和玉米基因组测序项目表明，转座子代表了这两种基因组的重要部分。在细胞水平上，转座子活性需要 DNA 和染色体修复过程来防止细胞损伤，从而导致细胞增殖失控。除了这些关于转座子活性后果的下游研究之外，对于体内调节其上游（切除和插入）行为的蛋白质知之甚少。这项研究将使用基于蛋白质组学的方法来识别调节转座行为的宿主蛋白，并可能识别调节细胞增殖的候选蛋白，并建立一种可行的病毒载体基因治疗替代方案。本项目的具体目标是：（1）比较Mu激活前后组织的蛋白质组； (2)细化转位机制的空间表达模式； (3) 挖掘基因组和 cDNA 数据库来识别移码翻译候选基因。将使用分子、生化和反向遗传学方法进一步分析四种选定组织类型中与转座相关的蛋白质的发育作用。

项目成果

Maize host requirements for Ustilago maydis tumor induction.

玉米黑粉菌肿瘤诱导的玉米宿主要求。

• DOI: 10.1007/s00497-009-0109-0
• 发表时间: 2010
• 期刊: Sexual plant reproduction
• 作者: Walbot,Virginia;Skibbe,DavidS
• 通讯作者: Skibbe,DavidS

Male reproductive development: gene expression profiling of maize anther and pollen ontogeny.

• DOI: 10.1186/gb-2008-9-12-r181
• 发表时间: 2008
• 期刊: GENOME BIOLOGY
• 影响因子: 12.3
• 作者: Ma, Jiong;Skibbe, David S.;Fernandes, John;Walbot, Virginia
• 通讯作者: Walbot, Virginia