The role of decellularised ECM proteins in the biological direction of ligament cells on a polymer/ECM scaffold

脱细胞 ECM 蛋白在聚合物/ECM 支架上韧带细胞生物学方向中的作用

• 批准号: 2866121
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2866121
• 关键词: role; decellularised; ECM; proteins; biological

Anterior cruciate ligament (ACL) injuries account for 40% of all sports-related procedures. Typically an autograft is used however these come with several disadvantages. Current synthetic ligaments have low clinical uptake due to failures. Electrospinning of degradable polymers such as PCL can be used to fabricate aligned structures that mimic the ligament architecture. However, while providing good physical and topographical cues they lack biological cues to stimulate matrix production. We hypothesise that by fabricating electrospun hybrids of degradable polymer and extracellular matrix (from decellularised ligament) these will provide both physical and biological cues and enhance ligament healing.Similar hybrids have been used for other applications, but not yet for ligament. Interestingly the literature shows that the addition of ECM powder increases the tensile strength of the polymer fibres. The mechanical properties of the synthetic ligaments are critical to their potential as a ligament repair technology, aswell as their ability to stimulate effective matrix production.

前交叉韧带（ACL）损伤占所有运动相关手术的40%。通常使用自体移植物，但这有几个缺点。目前的合成韧带由于失败，临床使用率很低。静电纺丝的可降解聚合物，如PCL可用于制造排列结构，模仿韧带的结构。然而，虽然提供了良好的物理和地形线索，但它们缺乏刺激基质产生的生物线索。我们假设，通过制造可降解聚合物和细胞外基质（来自脱细胞韧带）的电纺丝杂交体，这些将提供物理和生物线索，并促进韧带愈合。类似的混合材料已经用于其他应用，但还没有用于韧带。有趣的是，文献表明，ECM粉末的加入增加了聚合物纤维的抗拉强度。合成韧带的机械性能对其作为韧带修复技术的潜力以及刺激有效基质生成的能力至关重要。