TRANSGENIC & TARGETED MUTANT MICE PRESERVATION: BREEDING COLONIES: CRYOPRESERVE

转基因

• 批准号: 7391978
• 负责人: MURIEL T DAVISSON
• 金额: $ 145.98万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391978
• 关键词: TRANSGENIC; TARGETED; MUTANT; MICE; PRESERVATION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This competing renewal seeks continued funding for The Jackson Laboratory''''''''s (TJL) Induced Mutant Resource (IMR) to continue to provide this critical resource of transgenic and targeted mutation mice to the scientific community. Selectively altering the mouse genome by gene transfer (transgenic mice), homologous recombination and temporal- and tissue-specific production and use of induced mutant mice generated by these means has rapidly expanded, increasing the need for a resource to maintain, preserve, and distribute these mutant mice at a high health status and with minimal restrictions. Researchers frequently receive requests for mice they have created and want to believed of the burden that distributing mice to other scientists places on their research programs and grants. TJL, founded in 1929 as a non-profit research institution, has maintained and distributed mutant strains of mice for nearly 50 years. In 1992, TJL established the IMR whose purpose is to import, cryopreserve, and distribute biomedically important induced mutant mice. The need for the IMR is compelling and evidence by its rapid growth. Since it began, the IMR has accepted 495 mutant stocks, is distributing 299 of these, with another 58 currently in the importation process. In 1997, nearly 60,000 mice were distributed. We request support for rederivation, cryopreservation, and strain development, and strain development to improve the scientific value of strains in the IMR; mice are maintained for distribution to other scientists on as much of a cost recovery basis as possible. To improve the IMR resource program we propose to do research to: (1) develop methods to assure pathogen-free cyropreservation of mouse sperm, (2) phenotypically characterize new induced mutations and mutations transferred to new congenic genetic backgrounds, and (3) develop more cost-effective genotyping methods. Demand for IMR mice continues to increase, coincident with a decline in funding as several non-renewable start-up grants terminate. The ratio of rejected to accepted strains is increasing (see Introduction). The size of the need is beyond the scope of the NCRR alone to fund. NIAID and NIAMS already contribute to this effort by Intra-Agency Agreements. During the first 4 years of the current grant, over 70% of the mice supplied by the IMR were received by NIH intramural and extramural grantees of 16 NIH Institutes. To address the growing need we request approval of an expansion of this national resource for transgenic and targeted mice to authorize the NCRR to seek additional funding from NIH institutes. To aid in review we have subdivided the overall budget components. The Introduction explains the current funding mechanisms and our proposal to expand the IMR without an excessive burden to the NCRR.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这一竞争性的更新寻求继续为杰克逊实验室（TJL）诱导突变资源（IMR）提供资金，以继续为科学界提供转基因和靶向突变小鼠的关键资源。通过基因转移（转基因小鼠）、同源重组和时间特异性和组织特异性产生选择性改变小鼠基因组，以及通过这些手段产生的诱导突变小鼠的使用已经迅速扩大，增加了对资源的需求，以维持、保存和分配这些突变小鼠处于高健康状态并且具有最小的限制。研究人员经常收到要求他们创造的小鼠的请求，并希望相信将小鼠分配给其他科学家对他们的研究计划和赠款造成的负担。TJL成立于1929年，是一家非营利性研究机构，近50年来一直保持和分发突变小鼠品系。1992年，TJL建立了IMR，其目的是进口，冷冻保存和分发具有生物医学重要性的诱导突变小鼠。对IMR的需求是迫切的，其快速增长就是证据。自开始以来，IMR已经接受了495个突变种群，正在分发其中的299个，另有58个正在进口过程中。1997年，分发了近60 000只老鼠。我们请求支持再衍生、冷冻保存和菌株开发，以及菌株开发以提高IMR中菌株的科学价值;在尽可能多的成本回收基础上，将小鼠保留以分发给其他科学家。为了改善IMR资源计划，我们建议进行以下研究：（1）开发确保小鼠精子无病原体冷冻保存的方法，（2）表型表征新诱导突变和转移到新同源遗传背景的突变，以及（3）开发更具成本效益的基因分型方法。对IMR小鼠的需求继续增加，与此同时，随着一些不可再生的启动赠款终止，资金减少。被拒绝的菌株与被接受的菌株的比例正在增加（见引言）。需要的规模超出了国家难民和难民事务国家委员会单独供资的范围，国家机构间发展和监测研究所和国家机构间监测和评估研究所已经通过机构内协议为这一努力作出了贡献。在目前赠款的前4年，超过70%的小鼠由IMR提供的NIH内部和外部赠款的16个NIH研究所接收。为了满足日益增长的需求，我们请求批准扩大转基因和靶向小鼠的国家资源，以授权NCRR从NIH研究所寻求额外的资金。为了便于审查，我们对整个预算组成部分进行了细分。导言部分解释了目前的供资机制，以及我们关于扩大IMR而不给NCRR造成过度负担的建议。