Upgrade of X-Ray Diffractometers

X射线衍射仪升级

• 批准号: 6876388
• 负责人: PETER B. MOORE
• 金额: $ 34.41万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876388
• 关键词: X ray crystallography; biomedical equipment purchase; molecular /cellular imaging; optics; radioactive microsphere technique

DESCRIPTION (provided by applicant): Funds are requested so that two of the X-ray diffractometers used for macromolecular crystallography by investigators in the Yale Faculty of Arts and Sciences can be upgraded. The upgrade has two components: (1) replacement of a Rigaku RU300 rotating anode generator that currently supplies X-rays to both an R-Axis IV and a MAR 345 diffractometer, and (2) replacement of the associated X-ray optics. The RU300 generator in question, which was purchased in 1986, is increasingly prone to failure due to age, and even when operating properly, the X-ray beams it produces are not well matched to the crystallographic problems now being pursued, which involve smaller crystals than those usually studied in the 1980s, when the RU300 was purchased, and many of these crystals have large unit cell dimensions, which makes data collection even more challenging. The plan is to replace this generator with a Rigaku MicroMax-007 rotating anode generator. Not only should this generator be more reliable than the RU300 because it is newer, but because it is a microfocus generator, everything else being equal, its smaller, brighter beam should also enable investigators to collect diffraction data from almost all the crystals they are now studying much faster and more accurately than is now possible. In order to take full advantage of this MicroMax-007 generator the Osmic monochromators now mounted on the RU300 generator, which were built for generators that produce large beams, will have to be replaced with the VariMax optical components Rigaku recommends. Funds are requested for two such optical systems, so that both of the area detectors currently installed on the RU300 generator can be mounted on the new generator. It is anticipated that this upgrade will significantly increase the rate at which structures are determined at Yale.

描述（由申请人提供）：需要资金来升级耶鲁大学文理学院研究人员用于大分子晶体学的两台 X 射线衍射仪。此次升级包括两个部分：(1) 更换 Rigaku RU300 旋转阳极发生器，该发生器目前为 R-Axis IV 和 MAR 345 衍射仪提供 X 射线，(2) 更换相关的 X 射线光学器件。 所讨论的 RU300 发生器是 1986 年购买的，由于老化越来越容易出现故障，即使运行正常，它产生的 X 射线束也不能很好地匹配现在正在研究的晶体学问题，其中涉及的晶体比 1980 年代购买 RU300 时通常研究的晶体更小，而且许多晶体具有较大的晶胞尺寸，这使得数据收集更具挑战性。计划用 Rigaku MicroMax-007 旋转阳极发电机替换该发电机。该发生器不仅比 RU300 更可靠，因为它更新，而且因为它是微焦点发生器，在其他条件相同的情况下，其更小、更亮的光束还应该使研究人员能够比现在更快、更准确地从他们正在研究的几乎所有晶体中收集衍射数据。 为了充分利用 MicroMax-007 发生器，现在安装在 RU300 发生器上的 Osmic 单色仪（专为产生大光束的发生器而设计）必须更换为 Rigaku 推荐的 VariMax 光学元件。需要为两个这样的光学系统提供资金，以便当前安装在 RU300 发电机上的两个区域探测器都可以安装在新发电机上。 预计此次升级将显着提高耶鲁大学确定结构的速度。