Upgrade of X-Ray Diffractometers

X射线衍射仪升级

• 批准号: 6876388
• 负责人: PETER B. MOORE
• 金额: $ 34.41万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876388
• 关键词: X ray crystallography; biomedical equipment purchase; molecular /cellular imaging; optics; radioactive microsphere technique

DESCRIPTION (provided by applicant): Funds are requested so that two of the X-ray diffractometers used for macromolecular crystallography by investigators in the Yale Faculty of Arts and Sciences can be upgraded. The upgrade has two components: (1) replacement of a Rigaku RU300 rotating anode generator that currently supplies X-rays to both an R-Axis IV and a MAR 345 diffractometer, and (2) replacement of the associated X-ray optics. The RU300 generator in question, which was purchased in 1986, is increasingly prone to failure due to age, and even when operating properly, the X-ray beams it produces are not well matched to the crystallographic problems now being pursued, which involve smaller crystals than those usually studied in the 1980s, when the RU300 was purchased, and many of these crystals have large unit cell dimensions, which makes data collection even more challenging. The plan is to replace this generator with a Rigaku MicroMax-007 rotating anode generator. Not only should this generator be more reliable than the RU300 because it is newer, but because it is a microfocus generator, everything else being equal, its smaller, brighter beam should also enable investigators to collect diffraction data from almost all the crystals they are now studying much faster and more accurately than is now possible. In order to take full advantage of this MicroMax-007 generator the Osmic monochromators now mounted on the RU300 generator, which were built for generators that produce large beams, will have to be replaced with the VariMax optical components Rigaku recommends. Funds are requested for two such optical systems, so that both of the area detectors currently installed on the RU300 generator can be mounted on the new generator. It is anticipated that this upgrade will significantly increase the rate at which structures are determined at Yale.

描述（由申请人提供）：申请资金，以便在耶鲁大学艺术与科学学院的研究人员用于大分子晶体学的两个X射线衍射仪可以升级。此次升级包括两个部分：（1）更换理学RU 300旋转阳极发生器，该发生器目前为R-Axis IV和MAR 345衍射仪提供X射线;（2）更换相关的X射线光学器件。 所讨论的RU 300发生器是在1986年购买的，由于年龄的原因越来越容易发生故障，即使在正常运行时，它产生的X射线束也不能很好地与现在正在研究的晶体学问题相匹配，这些问题涉及比购买RU 300时的20世纪80年代通常研究的晶体更小的晶体，并且这些晶体中的许多晶体具有大的晶胞尺寸，这使得数据收集更具挑战性。计划用Rigaku MicroMax-007旋转阳极发生器替换该发生器。该发生器不仅比RU 300更可靠，因为它是新的，而且因为它是一个微焦点发生器，其他一切都是平等的，它更小，更亮的光束也应该使研究人员能够收集衍射数据，从几乎所有的晶体，他们现在正在研究比现在更快，更准确。 为了充分利用MicroMax-007发生器，现在安装在RU 300发生器上的Osmic单色器（用于产生大光束的发生器）必须更换为理学推荐的VariMax光学组件。请求为两个这样的光学系统提供资金，以便将目前安装在RU 300发电机上的两个区域探测器安装在新发电机上。 预计这一升级将大大提高耶鲁大学确定结构的速度。