ANTIBIOTIC RESISTANCE MUTATIONS IN H MARISMORTUI 50S RIBOSOMAL SUBUNITS

H MARISMORTUI 50S 核糖体亚基中的抗生素耐药性突变

• 批准号: 7726224
• 负责人: PETER B. MOORE
• 金额: $ 0.73万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-18 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7726224
• 关键词: 13-deoxytedanolide; Anisomycin; Antibiotic Resistance; Antibiotics; Binding Sites; Chloramphenicol; Computer Retrieval of Information on Scientific Projects Database; DNA Sequence Rearrangement; Distant; Drug Binding Site; Funding; Grant; Institution; Mutation; Object Attachment; Peptides; Pharmaceutical Preparations; Point Mutation; Protein Biosynthesis; RNA; Research; Research Personnel; Resistance; Resources; Source; Structure; Testing; United States National Institutes of Health; base; negamycin; novel; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structures of H. marismortui 50S ribosomal subunits containing antibitoic resistance mutations for chloramphenicol and anisomycin will be studied. The RNA point mutations do not occur immediately at the known binding sites for either drug, but occur one or more bases distant to the antibiotic binding site. Thus these experiments will test the hypothesis that subtle structural rearrangements may maintain features necessary for peptide bond formation but change the binding site for the drug. Novel antibiotics girolline, negamycin, and 13-deoxytedanolide, which have previously unidentified binding sites and unknown mechanisms of inhibiting protein synthesis termination will also be studied.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 将研究含有氯霉素和茴香霉素抗生素抗性突变的 H. marismortui 50S 核糖体亚基的结构。 RNA点突变不会立即发生在任一药物的已知结合位点处，而是发生在远离抗生素结合位点的一个或多个碱基处。 因此，这些实验将检验这样的假设：微妙的结构重排可能保持肽键形成所需的特征，但会改变药物的结合位点。 新型抗生素吉罗林（girolline）、奈加霉素（negamycin）和13-脱氧特那内酯（13-deoxytedanolide）也将被研究，这些抗生素具有先前未识别的结合位点和抑制蛋白质合成终止的未知机制。