NMR Console Upgrade and Cryoprobe

NMR 控制台升级和冷冻探针

• 批准号: 6440236
• 负责人: PETER B. MOORE
• 金额: $ 50万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440236
• 关键词: biomedical equipment purchase; nuclear magnetic resonance spectroscopy; structural biology

We request funding so that the console of the Varian Unity 500 MHz NMR spectrometer housed in the Yale Department of Molecular Biophysics and Biochemistry Bass Center can be replaced, and a cryoprobe purchased for that spectrometer. The Varian Inova console requested will replace a Unity console that was built ca. 1990, and was purchased by Yale, second hand, in 1995. Inova consoles have a more robust computer interface that requires fewer reboots, and that executes pulses, power level, phase changes and frequency changes more efficiently than Unity consoles. This upgrade should result in significantly improved implementations of pulse sequences. In addition, Inova consoles are compatible with Varian newest software, SPINCAD, which do3es not run on Unity or Unity plus systems. Thus this upgrade should ensure the usefulness of our 500 MHZ spectrometer for years to come. Last, but not least, replacement of the console in question will reduce the amount of instrument time lost for repair, which has become considerable because the Unity console is reaching the end of its useful life. The cryoprobe requested has recently been developed by Varian. Its major attraction is its high sensitivity, relative to room temperature probes. The sensitivity of the 500 MHz spectrometer equipped with a cryoprobe should be double the sensitivity observed with the best room temperature probe now available on the Yale 800 MHz spectrometer. There are several structural biology projects under consideration that demand this sensitivity because the molecules of interest aggregate at the concentrations required when standard probes are used.

我们要求提供资金，以便更换耶鲁大学分子生物物理学和生物化学系巴斯中心的Varian Unity 500 MHz NMR光谱仪的控制台，并为该光谱仪购买冷冻探针。要求的Varian Inova控制台将取代在CA. 1990年，被耶鲁大学收购，二手，1995年。Inova控制台具有更强大的计算机界面，需要更少的重新启动，并且比Unity控制台更有效地执行脉冲，功率电平，相位变化和频率变化。这一升级将导致脉冲序列的实施得到显著改善。此外，Inova控制台与瓦里安最新软件SPINCAD兼容，该软件不能在Unity或Unity plus系统上运行。因此，这次升级应确保我们的500兆赫光谱仪在未来几年的实用性。最后但并非最不重要的一点是，更换有问题的控制台将减少仪器的维修时间，由于Unity控制台即将达到其使用寿命，因此维修时间已经变得相当可观。Varian最近开发了所要求的冷冻探针。它的主要吸引力是它的高灵敏度，相对于室温探头。配备冷冻探针的500 MHz光谱仪的灵敏度应是Yale 800 MHz光谱仪上现有的最佳室温探针所观察到的灵敏度的两倍。有几个正在考虑的结构生物学项目需要这种灵敏度，因为当使用标准探针时，感兴趣的分子聚集在所需的浓度下。