COMPUTATIONAL TOOLS FOR RULE-BASED MODELING OF BIOCHEMICAL SYSTEMS

基于规则的生化系统建模的计算工具

• 批准号: 7254503
• 负责人: William S Hlavacek
• 金额: $ 28.01万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-09 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7254503
• 关键词: Accounting; Archives; Binding; Biochemical; Biochemical Reaction; Biological; Biological Models; Chemicals; Complex; Condition; Databases; Development; Electronics; Elements; Ensure; Equation; Goals; Graph; Hand; Human; Imagery; Individual; Knowledge; Maps; Methods; Modeling; Modification; Numbers; Play; Post-Translational Protein Processing; Procedures; Process; Property; Proteins; Range; Reaction; Resources; Role; Scheme; Signal Transduction; Site; Specific qualifier value; System; To specify; Visual; Work; base; biochemical model; combinatorial; computerized tools; mathematical model; protein protein interaction; software development; tool

DESCRIPTION (provided by applicant): A common feature of biochemical systems, especially those in which protein-protein interactions are prominent, is combinatorial complexity, which is present whenever a relatively small number of interactions have the potential to generate a much larger number of distinct chemical species and reactions. For a system marked by combinatorial complexity, the conventional approach of manually specifying each term of a mathematical model is impossible if the model is to account comprehensively for the consequences of interactions at the level of biomolecular sites. The primary goal of this proposal is to enable rapid development of mechanistic models of signal-transduction systems that account as completely as possible for the consequences of protein-protein interactions in a logically consistent way. To achieve this goal, we will develop tools for model specification and checking. These tools will be based on methods that involve the use of graphs to represent proteins and graph rewriting rules to represent protein-protein interactions. The rules are visual, much like diagrammatic interaction maps. Each rule specifies a type of binding/enzymatic reaction that arises from a biomolecular interaction and identifies features of reactants. Rules can be interpreted automatically, through procedures of graph rewriting, to obtain various types of mathematical models. Thus, rules enable precise and comprehensible visualization of biomolecular interactions. Importantly, a set of rules is compositional, in that each rule may be specified and refined independently. Equations in a conventional model on the other hand are typically interrelated, and changing an assumption about a protein-protein interaction may require numerous modifications of multiple equations. The second part of the proposed work is aimed at demonstrating the practicality of rule-based modeling. To ensure and demonstrate that our tools are useful, we will develop models for a number of biological systems. We will also demonstrate how our tools, together with database resources, can be used as part of a high- throughput modeling pipeline. An important capability we wish to achieve is the ability to model a significant fraction of the known human signal-transduction systems.

描述（由申请人提供）：生物化学系统的一个共同特征，特别是蛋白质-蛋白质相互作用突出的生物化学系统，是组合复杂性，每当相对少量的相互作用有可能产生大量不同的化学物质和反应时，就会出现组合复杂性。对于以组合复杂性为标志的系统，如果模型要全面考虑生物分子位点水平上相互作用的后果，则手动指定数学模型的每个项的常规方法是不可能的。该提案的主要目标是使信号转导系统的机械模型的快速发展，尽可能完整地解释蛋白质-蛋白质相互作用的结果在逻辑上一致的方式。为了实现这一目标，我们将开发用于模型规范和检查的工具。这些工具将基于涉及使用图来表示蛋白质和图重写规则来表示蛋白质-蛋白质相互作用的方法。这些规则是可视的，很像图解式的交互地图。每个规则指定了一种类型的结合/酶促反应，其产生于生物分子相互作用并识别反应物的特征。规则可以自动解释，通过程序的图形重写，以获得各种类型的数学模型。因此，规则使生物分子相互作用的精确和可理解的可视化成为可能。重要的是，一组规则是组合的，因为每个规则都可以独立地指定和细化。另一方面，常规模型中的方程通常是相互关联的，并且改变关于蛋白质-蛋白质相互作用的假设可能需要对多个方程进行多次修改。所提出的工作的第二部分是旨在证明基于规则的建模的实用性。为了确保和证明我们的工具是有用的，我们将开发一些生物系统的模型。我们还将演示如何将我们的工具与数据库资源一起用作高吞吐量建模管道的一部分。我们希望实现的一个重要能力是能够模拟已知的人类信号转导系统的重要部分。