INVESTIGATING NOVEL DNA FLUORSCENCE LABELING PROBES

研究新型 DNA 荧光标记探针

• 批准号: 7183279
• 负责人: KEVIN BURGESS
• 金额: $ 0.13万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7183279
• 关键词: biomedical resource

DESCRIPTION (adapted from the applicant's description): The thrust of the project is to develop and employ ground-breaking methods in the use of infrared spectroscopy for the study of biological macromolecules. Developments will include infrared analogues of NMR spectroscopy for the determination of the dynamics of structure change; heterodyned 2D and 3D IR methods for study of amide I and N-H stretch modes of proteins; pump/probe methods with IR pulses, and methods to determine energy transport between remote C=O and N-H or O-H groups in peptides and proteins; methods to measure and interpret IR photon echoes and vibrational frequency correlation functions essential for understanding the response functions needed for 2D IR; instrumentation to advance T-jumps associated with IR, and of mixing kinetic methods by multiplexing infrared detection; T-jumps and mixing methods using IR probes of the intermediates; immobilization techniques to examine equilibrium fluctuations and kinetics of proteins, and other assemblies such as cells, using single-molecule fluorescence methods; methods to study ultrafast light-induced processes in biological systems probed by optical and infrared methods. There are numerous collaborative projects designed to push the technologies being developed. These involve studies on reaction centers, rhodopsin and bacteriorhodopsin, the ribosome and t-RNA, LH2 single assemblies and terahertz band-to-band transitions, pentapeptide 2D IR, calmodulin, NO-myoglobin, 310 helicies, single coiled-coils, folding and unfolding of proteins, ?-helix 2D IR, interleukin 5, ?-interferon, single ion channels, cytochrome-c trehalose, prophyrin arrays, nitrile as a probe of conformations, triplet state singles, DNA base sequence probes, and rapid-mixing folding studies. There are service projects proposed in ultrafast optical and infrared, two-dimension and single -molecule spectroscopy, in areas of DNA helicases, protein folding, serpin-serine protease interaction, laser-induced pH juimps, porphyrin electon transfer, chemosensors, helix-coil transitions, ribonucleotide reductase, light-harvesting self assembly, and NorFES single peptide study. The Resource expects to publish two newsletters each year. Besides regular seminars they will hold a synmposium on multidimensional spectroscopies and a workshop in advances in infrared laser spectroscopy . The Resource will train visiting faculty researchers, post doctorals, and graduate and undergraduate students in the applicaitons of lasers to biology.

描述(改编自申请者的描述)：该项目的主旨是开发和使用突破性的方法来使用红外光谱来研究生物大分子。发展将包括用于确定结构变化动力学的核磁共振光谱的红外类似物；用于研究蛋白质的酰胺I和N-H伸缩模式的外差2D和3D IR方法；具有IR脉冲的泵浦/探针法，以及确定多肽和蛋白质中远程C=O和N-H或O-H基团之间能量传输的方法；测量和解释IR光子回波和振动频率关联函数的方法，这些方法对于理解2D IR所需的响应函数是必要的；推进与IR相关的T跳跃的仪器，以及通过多路红外检测混合动力学方法；使用中间体的IR探针的T跳跃和混合方法；使用单分子荧光方法检查蛋白质和其他组件(如细胞)的平衡波动和动力学的固定技术；通过光学和红外方法研究生物系统中超快光诱导过程的方法。 有许多合作项目旨在推动正在开发的技术。这些研究涉及反应中心、视紫红质和细菌视紫红质、核糖体和t-RNA、LH2单组装体和太赫兹带到带跃迁、五肽2D IR、钙调蛋白、非肌红蛋白、310螺旋、单螺旋线圈、蛋白质的折叠和去折叠、？-螺旋2D IR、白介素5、？-干扰素、单离子通道、细胞色素-c海藻糖、卟啉阵列、作为构象探针的腈、三态单态、DNA碱基序列探针和快速混合折叠研究。 在超快光学和红外、二维和单分子光谱学方面，在DNA螺旋酶、蛋白质折叠、丝氨酸-丝氨酸蛋白酶相互作用、激光诱导的pH调节、卟啉电子转移、化学传感器、螺旋-螺旋转变、核苷酸还原酶、捕光自组装和NorFeS单肽研究等领域提出了服务项目。 该资源预计每年出版两份时事通讯。除定期研讨会外，他们还将举办多维光谱仪专题讨论会和红外激光光谱学进展讲习班。该资源将培训访问学院的研究人员，博士后，以及研究生和本科生在激光在生物学中的应用。