DIFFUSION TENSOR MICROSCOPY OF STAINED MOUSE BRAIN

染色小鼠大脑的扩散张量显微镜

• 批准号: 7181509
• 负责人: BIN CHEN
• 金额: $ 0.53万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181509
• 关键词: bioimaging /biomedical imaging; biomedical resource; imaging /visualization /scanning; microscopy; technology /technique development

DESCRIPTION (provided by applicant): The enormous growth in the use of small animals for basic biomedical research has created a compelling need for new approaches to structural and functional phenotyping in the rodent. We propose continuation of the Duke Integrated Center for In Vivo Microscopy, an NIH/NCRR National Resource with an explicit focus on meeting this need. We propose four integrated cores that will provide researchers from around the world new image-based methods for structural and functional phenotyping using magnetic resonance microscopy (MRM). Core A: We will develop functional microscopy in the rodent to support cardiac motion analysis, pulmonary perfusion using arterial spin tagging and pulmonary ventilation using hypcrpolarized gas imaging. Core B: We will develop more efficient encoding strategies to increase our throughput for MR Histology by at least 500%. We will develop methods for actively staining fixed tissues for 3D MR histology. We will develop methods to measure regional neurodegeneration in the mouse brain. Core C: We will develop a new generation of technologies for physiologic support and monitoring of small animals permitting mutlimodality imaging (MRM. MicroPET, microCT, etc.) and image registration and long term studies on animals with compromised immunologic systems. Core D: We will develop visual informatics tools to permit effective archive, access, and analysis of very large multidimensional image sets from multiple modalities. The technologies will support a total of 16 collaborators anxious to use small animal imaging to extend the horizons of topics ranging from cancer to neurobiology. An aggressive education and dissemination program is included that will include undergraduate and graduate students, as well as post-doctoral fellows.

描述（由申请人提供）： 在基础生物医学研究中使用小动物的巨大增长已经产生了对啮齿动物结构和功能表型的新方法的迫切需求。我们建议继续杜克综合中心在体内显微镜，NIH/NCRR国家资源与明确的重点是满足这一需求。我们提出了四个集成的核心，将提供来自世界各地的研究人员使用磁共振显微镜（MRM）的结构和功能表型的新的基于图像的方法。核心A：我们将在啮齿动物中开发功能显微镜，以支持心脏运动分析，使用动脉自旋标记的肺灌注和使用超极化气体成像的肺通气。核心B：我们将开发更有效的编码策略，以将MR组织学的吞吐量提高至少500%。我们将开发用于3D MR组织学的主动染色固定组织的方法。我们将开发测量小鼠大脑中区域神经变性的方法。核心C：我们将开发新一代的技术，用于生理支持和监测小动物允许多模态成像（MRM）。MicroPET、microCT等）以及对免疫系统受损的动物的图像配准和长期研究。核心D：我们将开发可视化信息学工具，以允许有效的存档，访问和分析非常大的多维图像集从多种方式。这些技术将支持总共16个合作者，他们渴望使用小动物成像来扩展从癌症到神经生物学等主题的视野。一个积极的教育和传播计划包括将包括本科生和研究生，以及博士后研究员。