DIFFUSION TENSOR MICROSCOPY OF STAINED MOUSE BRAIN

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A new Duke Biomedical Engineering graduate student, Bin Chen, has taken over this project, to develop methods for identifying and displaying fiber tracks in the C57BL/6J mouse brain. We will merge the diffusion tensor studies with those of specimen staining to provide increased SNR (and therefore) increased spatial resolution. Staining Method: We propose two groups of 4 animals each, male C57BL/6J mice in the age range 2-6 months. Group A--controls will be perfused with 10% buffered formalin via a transcardial access. Group B--actively stained animals will be perfused with a 20:1 formalin/Prohance solution. Nota Bene: we have chosen to use a lower concentration of Prohance than is being used in ongoing staining projects so that the T2 is not shortened as much. Given the relatively long TE required for the heavey diffusion weighting, it seems reasonable to try to prepare specimens with slightly longer T2 even though the T1 will also be longer (~ 200 ms). Scanning method: 3D Diffusion Tensor Imaging with 13 different diffusion directions. Reduce encoding (by half) is applied after the first 3D reference image is acquired. Recon: Diffusion Tensor calculation