DIFFUSION TENSOR MICROSCOPY OF STAINED MOUSE BRAIN

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。杜克大学生物医学工程专业的一名新研究生陈斌接手了这个项目，开发出识别和显示C57BL/6J小鼠大脑中纤维轨迹的方法。我们将把扩散张量研究与标本染色的研究合并，以提供更高的SNR(因此)更高的空间分辨率。染色方法：我们提出两组动物，每组4只，雄性C57BL/6J小鼠，年龄2-6个月。A组--对照组将经心脏途径注入10%的缓冲福尔马林。B组：活动性染色动物，用20：1福尔马林/普朗斯溶液灌流。Nota Bene：我们选择使用比正在进行的染色项目中使用的浓度更低的Prohance，这样T2就不会缩短太多。鉴于Heavey扩散加权所需的相对较长的TE，即使T1也会更长(~200毫秒)，尝试制备T2稍长的样品似乎是合理的。扫描方法：13个不同扩散方向的三维扩散张量成像。在获取第一个3D参考图像之后应用缩减编码(减半)。RECON：扩散张量计算

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A new Duke Biomedical Engineering graduate student, Bin Chen, has taken over this project, to develop methods for identifying and displaying fiber tracks in the C57BL/6J mouse brain. We will merge the diffusion tensor studies with those of specimen staining to provide increased SNR (and therefore) increased spatial resolution. Staining Method: We propose two groups of 4 animals each, male C57BL/6J mice in the age range 2-6 months. Group A--controls will be perfused with 10% buffered formalin via a transcardial access. Group B--actively stained animals will be perfused with a 20:1 formalin/Prohance solution. Nota Bene: we have chosen to use a lower concentration of Prohance than is being used in ongoing staining projects so that the T2 is not shortened as much. Given the relatively long TE required for the heavey diffusion weighting, it seems reasonable to try to prepare specimens with slightly longer T2 even though the T1 will also be longer (~ 200 ms). Scanning method: 3D Diffusion Tensor Imaging with 13 different diffusion directions. Reduce encoding (by half) is applied after the first 3D reference image is acquired. Recon: Diffusion Tensor calculation