Mass spectrometry approaches to address capability gaps in drug discovery for membrane transporters

质谱方法可解决膜转运蛋白药物发现中的能力差距

• 批准号: 2877802
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2877802
• 关键词: Mass; spectrometry; approaches; address; capability

Transporters are a large family of proteins which convey sugars, amino acids, and other solutes across biological membranes. Some drugs target this family but in general transporters are sparsely exploited for therapeutic agents. This project aims to address two capability gaps in transporter drug discovery by applying state-of-the-art mass spectrometry (MS) techniques to detect solutes directly with the aim for assay development for drug discovery. The student will develop cell-based assays for membrane transporter function and apply them in the context of drug discovery projects for novel inhibitors with the industrial partner. Secondly, the student will develop MS-based whole-cell binding assays for transporters and other membrane proteins.

转运蛋白是一个大家族的蛋白质，其将糖、氨基酸和其他溶质运送穿过生物膜。一些药物靶向这一家族，但一般来说，转运蛋白很少用于治疗剂。该项目旨在通过应用最先进的质谱（MS）技术直接检测溶质来解决转运蛋白药物发现中的两个能力差距，目的是开发用于药物发现的测定方法。学生将开发基于细胞的膜转运蛋白功能测定，并将其应用于与工业合作伙伴合作的新型抑制剂药物发现项目的背景下。其次，学生将开发基于MS的转运蛋白和其他膜蛋白的全细胞结合试验。