A CAROLINA CENTER TO CHARACTERIZE & MAINTAIN MUTANT MICE: AIDS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 表示子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 关于艾滋病 描述（由申请人提供）： 由于人类和小鼠基因组的完成以及小鼠技术的进步，小鼠突变体和人类疾病模型的数量大幅增加。维持和分发无病原体的小鼠品系对于个体研究者来说既劳动密集又昂贵，以维持长期。NIH/NCRR认识到需要为转基因小鼠建立一个共同的储存库和配送中心，并于1999年资助了突变小鼠区域资源中心（MMRRC）。北卡罗来纳大学教堂山分校是四个MMRRC中心之一，正在开发这一资源，为整个生物医学界的利益。自1999年首次供资以来，已投入大量精力建立组织基础设施，并为突变小鼠品系的进口、再衍生、表型分析、冷冻保存和分配建立标准操作程序。在2001年，MMRRC-ESTA接受了它的第一个菌株，并经历了显着的扩展至今。在这些努力的同时，MMRRC还利用了在生物多样性委员会可用的专业知识，为社区带来新的技术和资源，如突变ES细胞系，gnotobiotic菌株和改进的体外受精方法。为了满足未来对MMRRC服务的需求并改进现有技术，该建议旨在：（1）进口、存档和分发转基因小鼠品系，（2）通过针对生物医学研究界的广告和招标提高MMRRC的知名度，（3）开发改进和可靠的辅助受精方法，（4）将无菌菌株开发整合到MMRRC服务中（5）在感兴趣的基因中产生等位基因系列的点突变作为社区资源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Regarding AIDS DESCRIPTION (provided by applicant): Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansion ever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.