A CAROLINA CENTER TO CHARACTERIZE & MAINTAIN MUTANT MICE: AIDS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 关于艾滋病 描述(由申请人提供)： 由于最近人类和小鼠基因组的完成以及小鼠技术的进步，小鼠突变和人类疾病模型的数量大幅增加。维持和分发无病原体的小鼠品系对个体研究人员来说既是劳动密集型的，也是长期维持的昂贵的。NIH/NCRR认识到需要一个通用的转基因小鼠储存库和分发中心，并在1999年做出回应，资助了突变小鼠区域资源中心(MMRRC)。北卡罗来纳大学教堂山分校是四个MMRRC中心之一，这些中心正在开发这种资源，以造福于整个生物医学界。自1999年最初提供资金以来，投入了大量精力来建立组织基础设施和建立标准操作程序(SOP)，用于突变小鼠品系的进口、再衍生、表型鉴定、冷冻保存和分配。2001年，MMRRC-UNC接受了它的第一个压力，并从那以后经历了显著的扩张。在这些努力的同时，MMRRC还利用了北卡罗来纳大学现有的专业知识，为社区带来了新的技术和资源，如突变的ES细胞系、诺生菌菌株和改进的体外受精方法。为了满足未来对MMRRC服务的需求并改进现有技术，这项建议旨在：(1)进口、存档和分发转基因小鼠品系；(2)通过针对生物医学研究社区的广告和征集来提高MMRRC的知名度；(3)开发改进的可靠的辅助受精方法；(4)将诺生菌菌株的开发整合到MMRRC服务中；(5)产生一系列感兴趣的基因的等位基因点突变，作为社区资源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Regarding AIDS DESCRIPTION (provided by applicant): Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansion ever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.