A Carolina Center to Characterize & Maintain Mutant Mice

卡罗来纳州中心的特色

• 批准号: 7909772
• 负责人: TERRY R MAGNUSON
• 金额: $ 24.66万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-04 至 2012-03-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7909772
• 关键词: Carolina; Center; Characterize; Maintain; Mutant

Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire .biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansionever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.

由于人类和小鼠基因组的完成以及小鼠基因组研究的进展， 随着技术的发展，小鼠突变体和人类模型的数量大幅增加， 疾病维持和分发无病原体的小鼠品系是劳动密集型的， 个人研究者要长期坚持。NIH/NCRR认识到需要一个共同的储存库 1999年，他们通过资助突变体来回应， 小鼠区域资源中心（MMRRC）。北卡罗来纳大学教堂山是四个MMRRC中心之一， 为整个生物医学界的利益开发这一资源。自1999年首次筹资以来， 在创建组织基础设施和建立 进口、再衍生、表型分析、冷冻保存和 突变小鼠品系的分布。在2001年，MMRRC-ESTA接受了它的第一个菌株， experienced经历significant-expansion显著-expansion扩张ever since以来.在作出这些努力的同时，妇女权利和儿童权利委员会还采取了 利用可利用的专业知识，为社区带来新的技术和资源， 作为突变ES细胞系，gnotobiotic菌株和改进的体外受精方法。以满足未来 为了满足MMRRC服务的需求，并改进现有技术，本提案旨在：（1）进口， 存档和分发转基因小鼠品系，（2）通过以下方式提高MMRRC的知名度 针对生物医学研究界的广告和招标，（3）开发改进的和可靠的 辅助受精的方法，（4）将无菌菌株的开发整合到MMRRC服务中（5） 在感兴趣的基因中产生一系列等位基因点突变作为社区资源。