A Carolina Center to Characterize & Maintain Mutant Mice

由于最近人类和小鼠基因组的完成以及小鼠的进展 随着科技的发展，老鼠突变体和人类模型的数量大幅增加。 疾病。维护和分发无病原体的小鼠品系既是劳动密集型的，也是昂贵的 个人调查人员，以维持长期。NIH/NCRR认识到需要一个共同的储存库 和转基因小鼠的配送中心，在1999年，他们通过资助突变人来回应 老鼠区域资源中心(MMRRC)北卡罗来纳大学-教堂山是四个MMRRC中心之一 开发这一资源，造福于整个生物医学界。自1999年首次资助以来， 已投入大量精力来创建组织基础设施和建立 标准操作程序(SOP)用于进口、再衍生、表型、冷冻保存和 突变小鼠品系的分布。2001年，MMRRC-UNC接受了它的第一个菌株，并 从那以后经历了显著的扩张。在这些努力的同时，MMRRC还采取了 利用北卡罗来纳大学现有的专业知识为社区带来新技术和资源，如 作为突变型ES细胞系、灵芝菌株和改良的体外受精方法。迎接未来 为了满足对MMRRC服务的需求并改进现有技术，该建议旨在：(1)进口， 存档和分发转基因小鼠品系，(2)通过以下方式提高MMRRC的可见度 针对生物医学研究社区的广告和征集，(3)开发改进和可靠的 辅助受精方法，(4)将诺生菌菌株开发纳入MMRRC服务(5) 在感兴趣的基因中产生一系列等位基因点突变，作为社区资源。

Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire .biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansionever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.