A Carolina Center to Characterize & Maintain Mutant Mice

由于人类和小鼠基因组的近期完成以及小鼠基因组研究的进展 随着技术的进步，小鼠突变体和人类模型的数量大幅增加 疾病。维护和分发无病原体的小鼠品系既费力又昂贵 个别调查员要维持长期。 NIH/NCRR 认识到需要一个公共存储库 转基因小鼠的配送中心，1999 年，他们的回应是资助突变体 小鼠区域资源中心 (MMRRC)。北卡罗来纳大学教堂山分校是四个 MMRRC 中心之一 开发该资源是为了整个生物医学界的利益。自 1999 年首次融资以来， 在创建组织基础设施和建立 用于导入、再衍生、表型分析、冷冻保存和 突变小鼠品系的分布。 2001 年，MMRRC-UNC 接受了第一株毒株，并已 此后经历了显着的扩张。在开展这些工作的同时，MMRRC 还采取了 利用北卡罗来纳大学现有的专业知识为社区带来新技术和资源，例如 如突变 ES 细胞系、限生菌株和改进的体外受精方法。去迎接未来 以满足 MMRRC 服务的需求并改进现有技术，该提案旨在：（1）导入、 存档并分发转基因小鼠品系，(2) 通过以下方式提高 MMRRC 的知名度： 针对生物医学研究界的广告和招标，(3) 开发改进和可靠的 辅助受精方法，(4) 将限菌菌株开发纳入 MMRRC 服务 (5) 在感兴趣的基因中生成一系列等位基因点突变作为社区资源。

Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire .biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansionever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.