A CAROLINA CENTER TO CHARACTERIZE & MAINTAIN MUTANT MICE

卡罗来纳州的特色中心

• 批准号: 8356889
• 负责人: TERRY R MAGNUSON
• 金额: $ 115.5万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8356889
• 关键词: A Mouse; Advertising; Archives; Biomedical Research; Communities; Cryopreservation; Development; ES Cell Line; Fertilization; Fertilization in Vitro; Funding; Future; Genes; Gnotobiotic; Grant; Human Genome; Individual; Methods; Mouse Strains; Mus; Mutant Strains Mice; National Center for Research Resources; Phenotype; Point Mutation; Principal Investigator; Procedures; Research; Research Infrastructure; Research Personnel; Resources; Series; Services; Source; Technology; United States National Institutes of Health; cost; experience; human disease; improved; interest; meetings; mouse genome; mutant; mutant mouse model; new technology; pathogen; repository

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DESCRIPTION (provided by applicant): Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansion ever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 描述（由申请人提供）： 由于人类和小鼠基因组的近期完成以及小鼠技术的进步，小鼠突变体和人类疾病模型的数量大幅增加。维持和分发无病原体的小鼠品系对于个别研究人员来说既是劳动密集型的，也是长期维持的成本高昂。 NIH/NCRR 认识到需要为转基因小鼠建立一个共同的储存库和分发中心，并于 1999 年通过资助突变小鼠区域资源中心 (MMRRC) 来做出回应。北卡罗来纳大学教堂山分校是四个 MMRRC 中心之一，这些中心正在开发这一资源，以造福整个生物医学界。自 1999 年首次资助以来，我们投入了大量精力来创建组织基础设施和建立突变小鼠品系的进口、再衍生、表型分析、冷冻保存和分发的标准操作程序 (SOP)。 2001 年，MMRRC-UNC 接受了第一批菌株，此后经历了显着的扩张。在这些努力的同时，MMRRC 还利用北卡罗来纳大学现有的专业知识，为社区带来新技术和资源，例如突变 ES 细胞系、无菌菌株和改进的体外受精方法。为了满足未来对 MMRRC 服务的需求并改进现有技术，本提案旨在：(1) 导入、存档和分发转基因小鼠品系，(2) 通过针对生物医学研究界的广告和招揽来提高 MMRRC 的知名度，(3) 开发改进且可靠的辅助受精方法，(4) 将限生菌株开发纳入 MMRRC 服务 (5) 在感兴趣的基因中生成一系列等位基因点突变作为社区资源。