600 MHZ NMR & PROBES: PROTEIN STRUCTURE, CNS

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：本申请是对宾夕法尼亚州立大学医学院（COM）的三通道600 MHz NMR光谱仪和常规脉冲梯度反向三重共振探针{1H，13 C，15 N}的部分资助的新请求。这种新仪器将补充最近订购的500 MHz频谱仪的COM校园，并共同提供国家的最先进的能力，以广泛的研究小组在这里COM以及几个小组从PSU主校区。医学院、生物化学和分子生物学系、药理学系、细胞和分子生理学系、癌症研究所和PSU哈克研究所（生命科学联盟）有35%的配套资金用于这些采购。以COM基金的形式提供额外的机构承诺，用于购买1H，13 C，{15 N}反向三重共振TCI冷冻探针，三年100%的工资支持，此后为生物化学和分子生物学系最近聘请的NMR设施经理提供50%的工资支持，并在第一年为仪器的运行提供全额资金。建议的600 MHz NMR和订购的500 MHz仪器将位于主楼（Crescent）的二楼，根据布鲁克OMNI服务部门的现场规划报告，这是最适合这些仪器的地方。除了NMR光谱仪的翻新需要外，还计划在周围的模块中进行额外的翻新，这将允许放置一些其他共享仪器，包括几个质谱仪，图形工作站和X射线晶体学设施。该委员会在管理和维护共享文书方面有着良好的记录。所要求的光谱仪将支持COM和PSU UP校园的研究工作，研究一系列医学相关蛋白质的蛋白质结构和动力学，以及COM中研究人脑和肌肉代谢通量的几个小组。冷冻探针技术已被证明为生物相关大分子的研究和代谢通量分析提供了显著的信噪比改善。这将使研究小组在这里和PSU主校区进行技术要求高的实验，如研究蛋白质的展开状态结构，确定大蛋白质或蛋白质复合物的结构，并使用13 C标记的代谢物跟踪代谢通量。此外，对于许多实验，冷冻探针提供了更高的吞吐量，这将加速NIH支持的研究项目，允许更多的研究人员使用该仪器。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application is a new request for partial funding of a three channel 600 MHz NMR spectrometer, and conventional pulse gradient inverse triple resonance probe {1H, 13C, 15N} in the Pennsylvania State University College of Medicine (COM). This new instrument will compliment the recently ordered 500 MHz spectrometer for the COM campus and together provide state-of-the-art capabilities to a wide range of research groups here at the COM as well as several groups from the PSU main campus. The College of Medicine, the Departments of Biochemistry and Molecular Biology, Pharmacology, Cellular and Molecular Physiology, the Cancer Institute, and the PSU Huck Institute (Life Sciences Consortium) have 35% matching funds for these purchases. An additional institutional commitment is provide in the form of COM funds to purchase a 1H, 13C, {15N} inverse triple resonance TCI cryoprobe, 100% salary support for three years and 50% salary support thereafter for the recently hired NMR facility manager in the Department of Biochemistry and Molecular Biology and full funding of the operation of the instrument during its first year. The proposed 600 MHz NMR and ordered 500 MHz instruments will be located on the second floor of the main building (Crescent) which was deemed the best suited for these instruments based upon a site planning report from the Bruker OMNI service. In addition to the renovations need the NMR spectrometers additional renovations are planned in the surrounding modules that will allow the placement of a number of other shared instruments including several mass spectrometers, graphics workstations, and X-ray crystallographic facility. The COM has an excellent record in the administration and upkeep of shared instruments. The requested spectrometer will support the research efforts in the COM and at the PSU UP campus studying protein structure and dynamics for a range of medically relevant proteins and also several groups in the COM studying metabolic flux in human brain and muscle. The cryoprobe technology has been demonstrated to provide a significant improvement in signal-to-noise ratios for studies of biological relevant macromolecules and for metabolic flux analysis. This will allow researcher groups here and at the PSU main campus to perform technically demanding experiments such as studying the unfolded state structure of proteins, determining the structures of large proteins or protein complexes, and following metabolic flux using 13C-labeled metabolites. In addition, for many experiments the cryoprobe provides increased throughput that will accelerate the NIH supported research projects allowing and allow a larger group of researches access to this instrument.