600 MHZ NMR & PROBES: PROTEIN STRUCTURE, CNS

600兆赫核磁共振

• 批准号: 7335062
• 负责人: John M Flanagan
• 金额: $ 10万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335062
• 关键词: X ray; biochemistry; brain; human; income; macromolecule; medicine; molecular biology; muscle; neoplasm /cancer; noise; pharmacology; physiology; protein structure; proteins; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application is a new request for partial funding of a three channel 600 MHz NMR spectrometer, and conventional pulse gradient inverse triple resonance probe {1H, 13C, 15N} in the Pennsylvania State University College of Medicine (COM). This new instrument will compliment the recently ordered 500 MHz spectrometer for the COM campus and together provide state-of-the-art capabilities to a wide range of research groups here at the COM as well as several groups from the PSU main campus. The College of Medicine, the Departments of Biochemistry and Molecular Biology, Pharmacology, Cellular and Molecular Physiology, the Cancer Institute, and the PSU Huck Institute (Life Sciences Consortium) have 35% matching funds for these purchases. An additional institutional commitment is provide in the form of COM funds to purchase a 1H, 13C, {15N} inverse triple resonance TCI cryoprobe, 100% salary support for three years and 50% salary support thereafter for the recently hired NMR facility manager in the Department of Biochemistry and Molecular Biology and full funding of the operation of the instrument during its first year. The proposed 600 MHz NMR and ordered 500 MHz instruments will be located on the second floor of the main building (Crescent) which was deemed the best suited for these instruments based upon a site planning report from the Bruker OMNI service. In addition to the renovations need the NMR spectrometers additional renovations are planned in the surrounding modules that will allow the placement of a number of other shared instruments including several mass spectrometers, graphics workstations, and X-ray crystallographic facility. The COM has an excellent record in the administration and upkeep of shared instruments. The requested spectrometer will support the research efforts in the COM and at the PSU UP campus studying protein structure and dynamics for a range of medically relevant proteins and also several groups in the COM studying metabolic flux in human brain and muscle. The cryoprobe technology has been demonstrated to provide a significant improvement in signal-to-noise ratios for studies of biological relevant macromolecules and for metabolic flux analysis. This will allow researcher groups here and at the PSU main campus to perform technically demanding experiments such as studying the unfolded state structure of proteins, determining the structures of large proteins or protein complexes, and following metabolic flux using 13C-labeled metabolites. In addition, for many experiments the cryoprobe provides increased throughput that will accelerate the NIH supported research projects allowing and allow a larger group of researches access to this instrument.

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：此申请是宾夕法尼亚州立大学医学院(COM)的三通道600 MHz核磁共振光谱仪和常规脉冲梯度反三重共振探头{1H，13C，15N}的新申请。这台新仪器将补充最近为COM园区订购的500 MHz光谱仪，并共同为COM的各种研究小组以及PSU主校园的几个小组提供最先进的功能。医学院、生物化学和分子生物学系、药理学、细胞和分子生理学系、癌症研究所和PSU哈克研究所(生命科学联盟)有35%的配套资金用于这些购买。一项额外的机构承诺以COM基金的形式提供，用于购买1H，13C，{15N}反三重共振TCI冷冻探头，三年100%工资支持，此后为生物化学和分子生物学系最近聘用的核磁共振设施经理提供50%工资支持，并为仪器第一年的运营提供全额资金。拟议的600 MHz核磁共振和订购的500 MHz仪器将位于主楼(新月)的二楼，根据Bruker Omni服务的现场规划报告，该建筑被认为是最适合这些仪器的。除了需要翻新核磁共振光谱仪外，还计划在周围的模块中进行额外的翻新，以允许放置一些其他共享仪器，包括几台质谱仪、图形工作站和X射线结晶学设施。COM在共享仪器的管理和维护方面有着出色的记录。所要求的光谱仪将支持COM和PSU UP园区的研究工作，研究一系列医学相关蛋白质的蛋白质结构和动力学，以及COM中的几个小组研究人类大脑和肌肉的代谢流量。低温探头技术已被证明为生物相关大分子的研究和代谢通量分析提供了显著的信噪比改进。这将允许这里和PSU主校区的研究小组进行技术要求苛刻的实验，例如研究蛋白质的未折叠状态结构，确定大型蛋白质或蛋白质复合体的结构，以及使用13C标记的代谢物跟踪代谢流量。此外，对于许多实验，低温探头提供了更高的吞吐量，这将加速NIH支持的研究项目，并允许更多的研究人员访问该仪器。