600 MHZ NMR & PROBES: PROTEIN STRUCTURE, PICONOVIRUS

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本申请是为宾夕法尼亚州立大学医学院（COM）的三通道600 MHz核磁共振光谱仪和传统脉冲梯度逆三重共振探针{1H， 13C， 15N}的部分资助提出的新申请。这台新仪器将补充最近为COM校园订购的500 MHz光谱仪，并共同为COM以及PSU主校区的多个研究小组提供最先进的能力。医学院、生物化学和分子生物学、药理学、细胞和分子生理学、癌症研究所和PSU哈克研究所（生命科学联盟）有35%的匹配资金用于这些购买。额外的机构承诺以COM资金的形式提供，用于购买1H， 13C， {15N}逆三共振TCI冷冻探针，为生物化学和分子生物学部门最近聘用的核磁共振设备经理提供三年100%的工资支持，此后提供50%的工资支持，并在第一年全额资助该仪器的运行。拟议的600 MHz NMR和订购的500 MHz仪器将位于主楼（Crescent）的二楼，根据布鲁克OMNI服务的场地规划报告，这被认为是最适合这些仪器的。除了对核磁共振光谱仪的改造外，还计划对周围的模块进行额外的改造，以便安置一些其他共享仪器，包括几个质谱仪、图形工作站和x射线晶体学设备。COM在管理和维护共享仪器方面有着出色的记录。所要求的光谱仪将支持COM和PSU UP校园的研究工作，研究一系列医学相关蛋白质的蛋白质结构和动力学，以及COM中的几个小组研究人脑和肌肉的代谢通量。冷冻探针技术已被证明为生物相关大分子研究和代谢通量分析提供了显着改善的信噪比。这将允许这里和PSU主校区的研究小组进行技术要求高的实验，例如研究蛋白质的未折叠状态结构，确定大蛋白质或蛋白质复合物的结构，以及使用13c标记的代谢物跟踪代谢通量。此外，对于许多实验，冷冻探针提供了更高的吞吐量，这将加速NIH支持的研究项目，并允许更大的研究小组使用该仪器。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application is a new request for partial funding of a three channel 600 MHz NMR spectrometer, and conventional pulse gradient inverse triple resonance probe {1H, 13C, 15N} in the Pennsylvania State University College of Medicine (COM). This new instrument will compliment the recently ordered 500 MHz spectrometer for the COM campus and together provide state-of-the-art capabilities to a wide range of research groups here at the COM as well as several groups from the PSU main campus. The College of Medicine, the Departments of Biochemistry and Molecular Biology, Pharmacology, Cellular and Molecular Physiology, the Cancer Institute, and the PSU Huck Institute (Life Sciences Consortium) have 35% matching funds for these purchases. An additional institutional commitment is provide in the form of COM funds to purchase a 1H, 13C, {15N} inverse triple resonance TCI cryoprobe, 100% salary support for three years and 50% salary support thereafter for the recently hired NMR facility manager in the Department of Biochemistry and Molecular Biology and full funding of the operation of the instrument during its first year. The proposed 600 MHz NMR and ordered 500 MHz instruments will be located on the second floor of the main building (Crescent) which was deemed the best suited for these instruments based upon a site planning report from the Bruker OMNI service. In addition to the renovations need the NMR spectrometers additional renovations are planned in the surrounding modules that will allow the placement of a number of other shared instruments including several mass spectrometers, graphics workstations, and X-ray crystallographic facility. The COM has an excellent record in the administration and upkeep of shared instruments. The requested spectrometer will support the research efforts in the COM and at the PSU UP campus studying protein structure and dynamics for a range of medically relevant proteins and also several groups in the COM studying metabolic flux in human brain and muscle. The cryoprobe technology has been demonstrated to provide a significant improvement in signal-to-noise ratios for studies of biological relevant macromolecules and for metabolic flux analysis. This will allow researcher groups here and at the PSU main campus to perform technically demanding experiments such as studying the unfolded state structure of proteins, determining the structures of large proteins or protein complexes, and following metabolic flux using 13C-labeled metabolites. In addition, for many experiments the cryoprobe provides increased throughput that will accelerate the NIH supported research projects allowing and allow a larger group of researches access to this instrument.