CRYOPROBE ACCESSORY FOR A 600 MHZ NMR SPECTROMETER: AIDS

600 MHZ NMR 波谱仪的冷冻探针配件：艾滋病

• 批准号: 7335033
• 负责人: NEPALLI RAMA KRISHNA
• 金额: $ 4.01万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-15 至 2007-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335033
• 关键词: AIDS; proteins

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The 600 MHz NMR Spectrometer (Bruker Avance-600) at the NMR Shared Facility of the University of Alabama at Birmingham currently provides the highest operating field available for the faculty members on the campus. It is being utilized by a diverse group of investigators working on projects ranging from the characterization of trace amounts of compounds requiring high sensitivity detection to the three dimensional structure determinations of proteins by 3D/4D-NMR spectroscopy. To significantly enhance the efficiency of this instrument in accommodating these research projects on biomolecular systems, a request is made for the purchase of a triple resonance inverse CryoProbe accessory for the 600 MHz NMR system. This probe provides a sensitivity enhancement of 300% to 400% (depending upon the sample) over the conventional room temperature probe, and will dramatically and significantly enhance the capabilities of the 600 MHz NMR spectrometer in meeting the needs of the UAB campus. With the significant improvement in sensitivity, the instrument can :(i) reduce data acquisition times for 3D/4D-NMR measurements on isotopically labeled proteins from several weeks or months to just a few days, (ii) increase the through-put of NMR samples, (iii) permit the study of biomolecules that are available only in low concentrations or only sparingly soluble, (iv) permit the structural study of proteins and compounds that have a limited life time, and (v) permit the structural characterization of proteins that tend to dimerize at mM concentrations but are predominantly monomeric at low concentrations. This 600 MHz CryoProbe upgrade will have a significant impact upon the programs of a major user group consisting of eight faculty members with research projects dealing with multidimensional NMR structural investigations on human apolipoprotein A-l, interferon-tau, cardiac muscle regulatory proteins, interaction of calmodulin with HIV gp41, bacteriophage P22 scaffolding protein, drug/DNA complexes, surface oligosaccahrides isolated from the Bacillus anthracis Exosporium, and the 1H and ISC-characterization of metabolic intermediates in low concentration.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：阿拉巴马大学伯明翰分校 NMR 共享设施中的 600 MHz NMR 波谱仪 (Bruker Avance-600) 目前为校园内的教职人员提供了最高的操作视野。它被不同的研究小组所使用，他们从事的项目包括从需要高灵敏度检测的痕量化合物的表征到通过 3D/4D-NMR 光谱法测定蛋白质的三维结构。为了显着提高该仪器适应这些生物分子系统研究项目的效率，要求购买用于 600 MHz NMR 系统的三重共振逆 CryoProbe 附件。与传统室温探头相比，该探头的灵敏度提高了 300% 至 400%（取决于样品），并将显着增强 600 MHz NMR 波谱仪的能力，满足 UAB 校园的需求。随着灵敏度的显着提高，该仪器可以：(i) 将同位素标记蛋白质的 3D/4D-NMR 测量数据采集时间从几周或几个月减少到几天，(ii) 增加 NMR 样品的通量，(iii) 允许研究仅以低浓度或仅微溶的生物分子，(iv) 允许研究蛋白质和 (v) 允许对在 mM 浓度下倾向于二聚化但在低浓度下主要为单体的蛋白质进行结构表征。此次 600 MHz CryoProbe 升级将对主要用户组的计划产生重大影响，该用户组由 8 名教员组成，其研究项目涉及人类载脂蛋白 A-1、干扰素 tau、心肌调节蛋白、钙调蛋白与 HIV gp41 的相互作用、噬菌体 P22 支架蛋白、药物/DNA 复合物、 从炭疽芽孢杆菌外孢子中分离的表面寡糖，以及低浓度代谢中间体的 1H 和 ISC 表征。