CRYOPROBE ACCESSORY FOR A 600 MHZ NMR SPECTROMETER: BIOCHEMISTRY, MICROBIOLOGY

600 MHZ NMR 波谱仪的冷冻探针配件：生物化学、微生物学

• 批准号: 7335035
• 负责人: NEPALLI RAMA KRISHNA
• 金额: $ 14.71万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-15 至 2007-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335035
• 关键词: biochemistry; proteins

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The 600 MHz NMR Spectrometer (Bruker Avance-600) at the NMR Shared Facility of the University of Alabama at Birmingham currently provides the highest operating field available for the faculty members on the campus. It is being utilized by a diverse group of investigators working on projects ranging from the characterization of trace amounts of compounds requiring high sensitivity detection to the three dimensional structure determinations of proteins by 3D/4D-NMR spectroscopy. To significantly enhance the efficiency of this instrument in accommodating these research projects on biomolecular systems, a request is made for the purchase of a triple resonance inverse CryoProbe accessory for the 600 MHz NMR system. This probe provides a sensitivity enhancement of 300% to 400% (depending upon the sample) over the conventional room temperature probe, and will dramatically and significantly enhance the capabilities of the 600 MHz NMR spectrometer in meeting the needs of the UAB campus. With the significant improvement in sensitivity, the instrument can :(i) reduce data acquisition times for 3D/4D-NMR measurements on isotopically labeled proteins from several weeks or months to just a few days, (ii) increase the through-put of NMR samples, (iii) permit the study of biomolecules that are available only in low concentrations or only sparingly soluble, (iv) permit the structural study of proteins and compounds that have a limited life time, and (v) permit the structural characterization of proteins that tend to dimerize at mM concentrations but are predominantly monomeric at low concentrations. This 600 MHz CryoProbe upgrade will have a significant impact upon the programs of a major user group consisting of eight faculty members with research projects dealing with multidimensional NMR structural investigations on human apolipoprotein A-l, interferon-tau, cardiac muscle regulatory proteins, interaction of calmodulin with HIV gp41, bacteriophage P22 scaffolding protein, drug/DNA complexes, surface oligosaccahrides isolated from the Bacillus anthracis Exosporium, and the 1H and ISC-characterization of metabolic intermediates in low concentration.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：位于伯明翰的亚拉巴马大学NMR共享设施的600 MHz NMR光谱仪（Bruker Avance-600）目前为校园内的教职员工提供了最高的操作场。它正在被一组不同的研究人员利用，这些研究人员正在进行从需要高灵敏度检测的痕量化合物的表征到通过3D/4D-NMR光谱法确定蛋白质的三维结构的项目。为了显著提高该仪器在适应这些生物分子系统研究项目方面的效率，要求购买用于600 MHz NMR系统的三重共振反向CryoProbe附件。这种探头提供了一个300%至400%的灵敏度提高（取决于样品）超过传统的室温探头，并将显着提高600 MHz NMR光谱仪的能力，以满足UAB校园的需求。随着灵敏度的显著提高，该仪器可以：（i）将同位素标记的蛋白质上的3D/4D-NMR测量的数据采集时间从几周或几个月减少到仅仅几天，（ii）增加NMR样品的通量，（iii）允许仅以低浓度或仅微溶的生物分子的研究，（iv）允许具有有限寿命的蛋白质和化合物的结构研究，以及（v）允许倾向于在mM浓度下二聚化但在低浓度下主要是单体的蛋白质的结构表征。这个600 MHz的CryoProbe升级将对一个主要用户组的计划产生重大影响，该用户组由八名教员组成，他们的研究项目涉及人类载脂蛋白A-I、干扰素-tau、心肌调节蛋白、钙调蛋白与HIV gp 41、噬菌体P22支架蛋白、药物/DNA复合物的相互作用的多维NMR结构研究，从炭疽芽孢杆菌外孢分离的表面寡糖，以及低浓度代谢中间体的1H和ISC表征。