800 MHz NMR Spectrometer for the Central Alabama High-Field NMR Facility

描述(由申请人提供)：一个多机构用户组正在请求从NCRR-HEI计划获得资金，用于建立一个由NIH-NCRR支持的具有低温探测器的800 MHz核磁共振光谱仪，作为进行生物分子研究的共享资源。用户群来自阿拉巴马州和密西西比州的六个机构，包括来自四个主要的博士授予机构(阿拉巴马大学伯明翰分校(UAB)、奥本大学、阿拉巴马大学(塔斯卡卢萨)和密西西比大学)和两个主要的非研究生机构(塔斯基吉大学和密西西比学院)的教职员工。该设施将实际位于牵头机构UAB的校园内，位置便利，便于其余机构的教职员工，并具有为多机构用户群体运营和管理这一共享资源的基础设施。UAB管理部门和几个部门和中心还在空间和初始运营成本方面对拟议的高场核磁共振设施做出了重大承诺。基于TROSY的脉冲序列在800 MHz线上缩窄效率的提高，将使这些机构中的一些机构能够对更大的蛋白质和蛋白质复合体进行结构研究，这些蛋白质和蛋白质复合体在现有的600 MHz和500 MHz核磁共振技术下是不适用的。更高的灵敏度(由CryoProbe进一步显著增强)和800 MHz的化学位移分散将使人们能够研究微量的化合物、难于溶解的蛋白质和复杂的碳水化合物，这些经常受到共振重叠的困扰。该800 MHz核磁共振系统将是拟议中的阿拉巴马州中部高场核磁共振设施的核心部分，并将对NIH支持的研究项目以及阿拉巴马州和密西西比州的其他项目产生重大影响。与公共健康相关：拟议的800 MHz核磁共振仪器将提供尖端研究能力，这对于对在疾病中发挥重要作用的生物分子的一些研究至关重要。它还将在表征低分子量天然产物和基于结构设计具有治疗这些疾病潜力的新化合物方面具有重要意义。

DESCRIPTION (provided by applicant): A multi-institutional user group is requesting funding from the NCRR-HEI Program for an 800 MHz NMR spectrometer with a CryoProbe to establish an NIH-NCRR-supported "Central Alabama High-Field NMR Facility" as a Shared Resource to conduct biomolecular research. The user group is drawn from six institutions in Alabama and Mississippi, and includes faculty members from four major PhD granting institutions (the University of Alabama at Birmingham (UAB), Auburn University, the University of Alabama (Tuscaloosa), and the University of Mississippi) and two predominantly undegraduate institutions (Tuskegee University and Mississippi College). The facility will be physically located on the campus of the lead insitution, UAB, which is conveniently located to the faculty members from the remaining institutions, and has the infra-structure in place for the operation and management of this Shared Resource for the multi-institutional user group. The UAB Administration and several Departments and Centers have also made a significant commitment to this proposed High-Field NMR Facility in terms of space and the initial operating costs. The increased efficiency at 800 MHz in line narrowing for TROSY-based pulse sequences will enable structural investigation of larger proteins and protein complexes not amenable at existing 600 MHz and 500 MHz NMR technology at some of these institutions. The higher sensitivity (further significantly enhanced by the CryoProbe) and chemical shift dispersion at 800 MHz will enable investigations on trace quantities of compounds, sparingly soluble proteins, and complex carbohydrates which are often plagued by resonance overlap. This 800 MHz NMR system will be the center piece of the proposed Central Alabama High-Field NMR Facility and will have a significant impact on NIH- supported research projects as well as other projects in the States of Alabama and Mississippi. PUBLIC HEALTH RELEVANCE: The proposed 800 MHz NMR instrument will provide cutting-edge research capability that is critical for a number of investigations on biomolecules that play a significant role in diseases. It will also be significant in the characterization of low molecular weight natural products and the structure-based design of new compounds with therapeutic potential in treating these diseases.