X-RAY CRYSTALLOGRAPHY

X射线晶体学

• 批准号: 7671825
• 负责人: NIKOLA P PAVLETICH
• 金额: $ 15.75万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7671825
• 关键词: Address; Antineoplastic Agents; Arts; Biological Process; Cells; Complex; Computer software; Crystallography; Data; Data Collection; Decompression Sickness; Diagnostic radiologic examination; Equipment; Genes; Genomics; Goals; Housing; Institution; Laboratories; Location; Malignant Neoplasms; Memorial Sloan-Kettering Cancer Center; Methods; Modeling; Molecular; Molecular Structure; Play; Process; Protein-Protein Interaction Map; Publications; Range; Regulation; Research; Research Personnel; Resources; Roentgen Rays; Role; Running; Staging; Structure; Synchrotrons; Therapeutic; Time; Training; X-Ray Crystallography; beamline; cell behavior; data acquisition; design; drug discovery; macromolecular assembly; programs; research and development; research study; scaffold; structural biology; tool

The primary goal of the X-ray Crystallography Core is to enable and facilitate the research of MSKCC laboratories that use X-ray crystallography as a tool to address questions in their research programs. The facility maintains in-house equipment for data collection, processing and structure determination, implements a wide range of crystallographic and structure analysis software packages, provides long-term regular access to state-of-the-art synchrotron beamlines through participation in multi-institutional consortia at two national laboratory locations, provides training and technical assistance to users of both the in-house and remote facilities, and provides expertise in structural biology and modeling and guidance to non-structural MSKCC laboratories that benefit from the use of available structures in the design and interpretation of experiments. Structural biology has been playing an increasing role in understanding the many biological processes important in cancer and in accelerating the pace of anti-cancer drug discovery. In the post-genomic era, the need for structural data to understand biological function and regulation, to provide scaffolds for the design or improvement of candidate anticancer compounds, and to help identify molecular function will increase with the application of massively parallel data acquisition tools such as DMA arrays, global protein-protein interaction maps and large scale identification of cancer-related genes. This will require increased throughput from conventional X-ray crystallography facilities, and new, state-of-the-art synchrotron facilities to accelerate structure determination and to allow the study of increasingly large and complex structures and macromolecular assemblies.

X射线晶体学核心的首要目标是使MSKCC的研究成为可能和便利 使用X射线结晶学作为工具来解决其研究计划中的问题的实验室。这个 设施维护用于数据收集、处理和结构确定的内部设备，实施 种类繁多的结晶学和结构分析软件包，长期定期提供 通过参与两个国家的多机构联合体获得最先进的同步辐射光束线 国家实验室地点，为内部和 远程设施，并提供结构生物学和建模方面的专业知识，并为非结构性 MSKCC实验室，受益于在设计和解释中使用可用的结构 实验。 结构生物学在理解许多生物过程方面发挥着越来越大的作用。 在癌症和加快抗癌药物发现的步伐方面具有重要意义。在后基因组时代， 需要结构数据来了解生物功能和调节，为设计提供支架 或改进候选抗癌化合物，并帮助识别分子功能将随着 DMA阵列、Global Protein-Protein等大规模并行数据采集工具的应用 癌症相关基因的互作图谱和大规模鉴定。这将需要增加 来自传统X射线结晶学设备和新的、最先进的同步加速器设备的吞吐量 为了加快结构确定，并允许研究越来越大和复杂的结构和 大分子组装。