Acquisition of a Bruker X8 Prospector Protein X-ray Crystallography System

采购 Bruker X8 Prospector 蛋白质 X 射线晶体学系统

• 批准号: 7596030
• 负责人: THOMAS L POULOS
• 金额: $ 47.73万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-07 至 2010-05-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7596030
• 关键词: 11 year old; Air; Anabolism; Animal Model; Anodes; Antibiotics; Antibodies; Apoptosis; California; Communities; Computer software; Crystallization; Crystallography; Data; Data Collection; Development; Drops; Drug Delivery Systems; Enzymes; Equipment; Faculty; Funding; Goals; Grant; Health; Housing; Individual; Investigation; Maintenance; Nitric Oxide Synthase; Operative Surgical Procedures; Proteins; Recruitment Activity; Request for Proposals; Roentgen Rays; Running; Site; Source; Structural Biologist; Structure; Synchrotrons; System; Testing; Tube; United States National Institutes of Health; Universities; Viral Genome; Virus; X-Ray Crystallography; base; cost; design; instrument; public health relevance; seal; structural biology; tuberculosis drugs; user-friendly

DESCRIPTION (provided by applicant): This proposal requests funds for purchase of a new X-ray data collection system to serve as the primary data collection instrument for macromolecular crystallography at the University of California, Irvine. The current system is 11 years old and has a high yearly cost to maintain. Even though synchrotron data collection is extensively used by the UCI community, there is still the need for a reliable in house system to screen crystals prior to synchrotron trips, search for heavy atom derivatives, and in some cases solve structures using in house data. Our goal is to replace the older system with a highly reliable system that is inexpensive to run. We propose to purchase a Bruker X8 Prospector CCD system equipped with a Incoatec microfocus sealed, air- cooled x-ray tube and 2D Montel multilayer mirrors. Since this system uses a sealed tube the X-ray source is essentially maintenance free as opposed to a rotating anode. We expect the yearly cost of operation to drop form ~$30,000 per year to ~$5,000 per year. There are 5 Major Users (Goulding, McPherson, Tsai, Poulos, and Sevrioukova) four of whom are funded by NIH grants. Dr. Goulding has been at UCI for only one year and will be applying for NIH support. Since 1992 when Prof. Poulos was recruited to UCI to initiate the development of structural biology at UCI, structural biology has substantially expanded. There are now 6 faculty crystallographers, 2 NMR spectroscopists, and 3 computational structural biologists with one more tow join UCI this summer. Given that crystallographic software, data collection, and crystallization have become much more user-friendly, the scope of crystallographic projects also has extended to many other labs that are not formally crystallographic labs. Dozens of protein, enzyme, virus and antibody structures have been solved, and the results have led to new molecule design and synthesis and animal model tests. Therefore, while this proposal has 5 primary users, there are 6 other faculty labs that also use the current system many of whom are funded by NIH grants. The requested equipment will provide the primary on site data collection system for 10 individual NIH funded projects. PUBLIC HEALTH RELEVANCE: Crystal structures of important health related proteins under investigation in the labs of the Major Users provide essential information for understanding function. The structural organization of the viral genome (McPherson), the structural biology of antibiotic biosynthesis (Tsai) the structural biology of TB drug targets (Goulding), structure based inhibition of nitric oxide synthase (Poulos) and mechanisms of apoptosis (Sevrioukova) all have clearly defined health related significance.

描述(由申请人提供)：该提案申请资金用于购买新的X射线数据收集系统，作为加州大学欧文分校大分子结晶学的主要数据收集仪器。目前的系统已有11年的历史，每年的维护成本很高。尽管同步加速器数据收集被UCI社区广泛使用，但仍然需要一个可靠的内部系统来在同步加速器运行之前筛选晶体，搜索重原子衍生物，并在某些情况下解决使用内部数据的结构。我们的目标是用运行成本低、高度可靠的系统取代较旧的系统。我们建议购买一台Bruker X8 Prospector CCD系统，配备Incoatec微焦密封、风冷X射线管和2D Montel多层反射镜。由于该系统使用密封管，因此X射线源基本上是免维护的，而不是旋转的阳极。我们预计每年的运营成本将从每年约30,000美元降至约5,000美元。有5个主要用户(Goulding、McPherson、Tsai、Poulos和Sevrioukova)，其中4个由NIH赠款资助。Goulding博士在UCI只工作了一年，将申请NIH的支持。自1992年Poulos教授受聘到UCI开始在UCI发展结构生物学以来，结构生物学已经有了很大的发展。现在有6名教师结晶学家，2名核磁共振光谱学家，3名计算结构生物学家，还有一名在今年夏天加入UCI。鉴于结晶学软件、数据收集和结晶已经变得更加人性化，结晶学项目的范围也扩展到许多其他非正式结晶学实验室的实验室。数十种蛋白质、酶、病毒和抗体的结构已经被解决，结果导致了新的分子设计和合成以及动物模型试验。因此，虽然这项提案有5个主要用户，但还有6个其他教师实验室也使用当前的系统，其中许多是由NIH拨款资助的。所需设备将为美国国立卫生研究院资助的10个项目提供主要的现场数据收集系统。与公共健康相关：主要使用者实验室正在研究的与健康有关的重要蛋白质的晶体结构为了解其功能提供了基本信息。病毒基因组的结构组织(McPherson)、抗生素生物合成的结构生物学(TSAI)、结核病药物靶标的结构生物学(Goulding)、基于结构的一氧化氮合酶抑制(Poulos)和细胞凋亡机制(Sevrioukova)都清楚地定义了与健康有关的意义。