Liquid Chromatography Mass Spectrometry System

描述（由申请人提供）：本申请资金用于购买沃茨Micromass液相色谱Quattro Premier串联四极杆质谱系统（LC/MS/MS），供Rutgers大学库克学院的5名NIH支持的研究者使用。购买该仪器沿着罗格斯大学的机构支持，将允许建立一个核心设施，为NIH资助的该研究小组的研究（以及罗格斯大学内外的其他研究）提供分析支持。这组研究人员的所有资助研究都集中在脂质代谢和作用以及脂质在维持最佳健康和预防疾病中的作用。细胞内和细胞外脂质是许多慢性疾病的重要因素，包括糖尿病、心血管疾病、肥胖、癌症、中风、关节炎和阿尔茨海默病，我们研究小组的研究最终旨在了解其中许多疾病。LC/MS/MS及其核心设施将提供对细胞、组织和其他实验样品中的脂质及其生物活性代谢物进行非常灵敏的定性和定量分析的能力。此外，LC/MS/MS将允许在培养细胞和动物模型中进行稳定同位素示踪动力学研究，以识别功能不同的脂质池，并研究脂质如何通过代谢途径转化为特定的生物活性或非活性终产物。这些用途将大大有利于我们NIH资助的用户组的研究能力，并将允许增加对脂质如何在保持良好健康和疾病病理学方面发挥作用的理解。罗格斯大学没有可用于这项研究的类似仪器。

DESCRIPTION (provided by applicant): This application requests funds for the purchase of a Waters Micromass Liquid Chromatograph Quattro Premier Tandem Quadrupole Mass Spectrometry System (LC/MS/MS) for use by a group of 5 NIH- supported investigators at Cook College of Rutgers University. The purchase of this instrument along with institutional support from Rutgers University will allow for the establishment of a core facility that will provide analytical support for the NIH-funded research of this investigator group (and for others from within and outside of Rutgers University). All of the funded research of this group of investigators is focused on lipid metabolism and actions and the role of lipids in maintaining optimal health and preventing disease. Intra- and extracellular lipids are important factors in many chronic diseases, including diabetes, cardiovascular disease, obesity, cancer, stroke, arthritis, and Alzheimer's disease and the research of our investigator group ultimately is aimed at understanding many of these diseases. The LC/MS/MS and the core facility housing it will provide the capability to carry out very sensitive qualitative and quantitative analyses of lipids and their bioactive metabolites in cells, tissues and other experimental samples. Additionally, the LC/MS/MS will allow for the undertaking of stable isotope tracer kinetic studies in cultured cells and animal models to identify functionally distinct lipid pools and to investigate how lipids are channeled metabolically to specific bioactive or inactive end products. These uses will benefit significantly the research capacity of our NIH-funded user group and will allow for increased understanding of how lipids act in maintaining good health and pathologically in disease. There are no comparable instruments at Rutgers University that are available for this research.