LIQUID CHROMATOGRAPHY MASS SPECT LIPID & DIABETES, CVD, OBESITY

液相色谱质谱脂质体

• 批准号: 7335054
• 负责人: Judith Storch
• 金额: $ 4.25万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-04 至 2007-04-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335054
• 关键词: health; lipids; liquid chromatography; obesity; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application requests funds for the purchase of a Waters Micromass Liquid Chromatograph Quattro Premier Tandem Quadrupole Mass Spectrometry System (LC/MS/MS) for use by a group of 5 NIH- supported investigators at Cook College of Rutgers University. The purchase of this instrument along with institutional support from Rutgers University will allow for the establishment of a core facility that will provide analytical support for the NIH-funded research of this investigator group (and for others from within and outside of Rutgers University). All of the funded research of this group of investigators is focused on lipid metabolism and actions and the role of lipids in maintaining optimal health and preventing disease. Intra- and extracellular lipids are important factors in many chronic diseases, including diabetes, cardiovascular disease, obesity, cancer, stroke, arthritis, and Alzheimer's disease and the research of our investigator group ultimately is aimed at understanding many of these diseases. The LC/MS/MS and the core facility housing it will provide the capability to carry out very sensitive qualitative and quantitative analyses of lipids and their bioactive metabolites in cells, tissues and other experimental samples. Additionally, the LC/MS/MS will allow for the undertaking of stable isotope tracer kinetic studies in cultured cells and animal models to identify functionally distinct lipid pools and to investigate how lipids are channeled metabolically to specific bioactive or inactive end products. These uses will benefit significantly the research capacity of our NIH-funded user group and will allow for increased understanding of how lipids act in maintaining good health and pathologically in disease. There are no comparable instruments at Rutgers University that are available for this research.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：此申请要求购买水的资金，用于购买水液色谱Quattro Quattro Quattro Premier Tandem Quadrupole质谱系统（LC/MS/MS），以供一组5名NIH支持的Rutgers University的NIH支持的调查员。购买该工具以及罗格斯大学的机构支持将允许建立一个核心设施，该设施将为该研究者组的NIH资助研究（以及罗特大学内外的其他人）提供分析支持。这组研究人员的所有资助研究都集中在脂质代谢和作用上，以及脂质在维持最佳健康和预防疾病中的作用。细胞内和细胞外脂质是许多慢性疾病的重要因素，包括糖尿病，心血管疾病，肥胖，癌症，癌症，中风，关节炎和阿尔茨海默氏病，以及我们研究人员组的研究最终旨在了解其中许多疾病。 LC/MS/MS及其核心设施壳将提供对细胞，组织和其他实验样品中脂质及其生物活性代谢物的非常敏感的定性和定量分析的能力。此外，LC/MS/MS将允许在培养的细胞和动物模型中进行稳定的同位素示踪剂动力学研究，以识别功能上不同的脂质池，并研究如何将脂质代谢转向特定的生物活性或无活性最终产物。这些用途将显着受益于我们NIH资助的用户组的研究能力，并可以加深对脂质在保持良好健康和病理上如何在疾病中采取良好健康的了解。罗格斯大学没有可比的乐器可用于这项研究。