LIQUID CHROMATOGRAPHY MASS SPECT: LIPID & CANCER

液相色谱质谱：脂质

• 批准号: 7335055
• 负责人: Judith Storch
• 金额: $ 4.25万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-04 至 2007-04-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335055
• 关键词: health; lipids; liquid chromatography; neoplasm /cancer; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application requests funds for the purchase of a Waters Micromass Liquid Chromatograph Quattro Premier Tandem Quadrupole Mass Spectrometry System (LC/MS/MS) for use by a group of 5 NIH- supported investigators at Cook College of Rutgers University. The purchase of this instrument along with institutional support from Rutgers University will allow for the establishment of a core facility that will provide analytical support for the NIH-funded research of this investigator group (and for others from within and outside of Rutgers University). All of the funded research of this group of investigators is focused on lipid metabolism and actions and the role of lipids in maintaining optimal health and preventing disease. Intra- and extracellular lipids are important factors in many chronic diseases, including diabetes, cardiovascular disease, obesity, cancer, stroke, arthritis, and Alzheimer's disease and the research of our investigator group ultimately is aimed at understanding many of these diseases. The LC/MS/MS and the core facility housing it will provide the capability to carry out very sensitive qualitative and quantitative analyses of lipids and their bioactive metabolites in cells, tissues and other experimental samples. Additionally, the LC/MS/MS will allow for the undertaking of stable isotope tracer kinetic studies in cultured cells and animal models to identify functionally distinct lipid pools and to investigate how lipids are channeled metabolically to specific bioactive or inactive end products. These uses will benefit significantly the research capacity of our NIH-funded user group and will allow for increased understanding of how lipids act in maintaining good health and pathologically in disease. There are no comparable instruments at Rutgers University that are available for this research.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本申请请求资金购买 Waters Micromass 液相色谱仪 Quattro Premier 串联四极杆质谱系统 (LC/MS/MS)，供罗格斯大学库克学院 5 名 NIH 支持的研究人员使用。购买该仪器以及罗格斯大学的机构支持将允许建立一个核心设施，为该研究小组（以及罗格斯大学内外的其他人）的 NIH 资助研究提供分析支持。该研究小组所有资助的研究都集中在脂质代谢和作用以及脂质在维持最佳健康和预防疾病方面的作用。细胞内和细胞外脂质是许多慢性疾病的重要因素，包括糖尿病、心血管疾病、肥胖、癌症、中风、关节炎和阿尔茨海默病，我们研究小组的研究最终旨在了解其中许多疾病。 LC/MS/MS 及其核心设施将能够对细胞、组织和其他实验样品中的脂质及其生物活性代谢物进行非常灵敏的定性和定量分析。此外，LC/MS/MS 将允许在培养细胞和动物模型中进行稳定同位素示踪动力学研究，以识别功能上不同的脂质库，并研究脂质如何通过代谢途径转化为特定的生物活性或非活性最终产品。这些用途将极大地提高我们 NIH 资助的用户组的研究能力，并将有助于加深对脂质在维持良好健康和疾病病理方面的作用的了解。罗格斯大学没有可用于这项研究的类似仪器。