LIQUID CHROMATOGRAPHY MASS SPECT: LIPID & CANCER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：本申请申请资金用于购买Waters微质谱液相色谱四极串联四极杆质谱仪(LC/MS/MS)，供罗格斯大学库克学院5名研究人员使用。购买这一仪器，再加上罗格斯大学的机构支持，将允许建立一个核心设施，为该研究小组由国家卫生研究院资助的研究(以及罗格斯大学内外的其他研究)提供分析支持。这组研究人员资助的所有研究都集中在脂类代谢和作用，以及脂类在维持最佳健康和预防疾病中的作用。细胞内和细胞外脂质是许多慢性疾病的重要因素，包括糖尿病、心血管疾病、肥胖、癌症、中风、关节炎和阿尔茨海默病，我们研究小组的研究最终旨在了解这些疾病中的许多。LC/MS/MS及其核心设施将提供对细胞、组织和其他实验样品中的脂类及其生物活性代谢物进行非常灵敏的定性和定量分析的能力。此外，LC/MS/MS将允许在培养细胞和动物模型中进行稳定的同位素示踪动力学研究，以确定不同功能的脂类池，并研究脂类如何通过代谢途径输送到特定的生物活性或非活性最终产品。这些用途将极大地提高我们NIH资助的用户群体的研究能力，并将使人们更好地了解血脂在维持良好健康和疾病病理方面的作用。罗格斯大学没有类似的仪器可用于这项研究。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application requests funds for the purchase of a Waters Micromass Liquid Chromatograph Quattro Premier Tandem Quadrupole Mass Spectrometry System (LC/MS/MS) for use by a group of 5 NIH- supported investigators at Cook College of Rutgers University. The purchase of this instrument along with institutional support from Rutgers University will allow for the establishment of a core facility that will provide analytical support for the NIH-funded research of this investigator group (and for others from within and outside of Rutgers University). All of the funded research of this group of investigators is focused on lipid metabolism and actions and the role of lipids in maintaining optimal health and preventing disease. Intra- and extracellular lipids are important factors in many chronic diseases, including diabetes, cardiovascular disease, obesity, cancer, stroke, arthritis, and Alzheimer's disease and the research of our investigator group ultimately is aimed at understanding many of these diseases. The LC/MS/MS and the core facility housing it will provide the capability to carry out very sensitive qualitative and quantitative analyses of lipids and their bioactive metabolites in cells, tissues and other experimental samples. Additionally, the LC/MS/MS will allow for the undertaking of stable isotope tracer kinetic studies in cultured cells and animal models to identify functionally distinct lipid pools and to investigate how lipids are channeled metabolically to specific bioactive or inactive end products. These uses will benefit significantly the research capacity of our NIH-funded user group and will allow for increased understanding of how lipids act in maintaining good health and pathologically in disease. There are no comparable instruments at Rutgers University that are available for this research.