BECTON-DICKINSON FACSARIA FLOW CYTOMETER: LUNG CANCER, OVARIAN CANCER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：本建议书建议将Becton-Dickinson(BD)FACSAria细胞分选机安置在西弗吉尼亚大学的流式细胞仪核心设施中。流式细胞术核心设施支持来自健康科学中心、玛丽·巴布·兰道夫癌症中心和其他地区校园的基础科学部的研究人员。该机构是3年前建立的，是国家研究资源中心授予的生物医学研究卓越中心的一部分，在使用和对额外服务的需求方面经历了大幅增长。仪器目前包括BD FACSCalibur和BD FACStarPlus细胞分选器。虽然FACSCalibur是最先进的，但分选机已有17年以上的历史，由于其老化而难以维护，并且数据采集和细胞分选能力有限。收购BD FACSAria将提供&gt；9参数细胞分析和高通量细胞分选，包括使用Hoechst染料基于细胞周期进行分选。改进的环境控制和生物危害控制也将有助于从受感染的动物和人类细胞中分离细胞，这将显著提高该设施的能力。新的仪器将在几个切实的方面使HSC受益，包括加强努力，将Mary Babb Randolph癌症中心提升为NCI指定的临床癌症中心，改善正在进行的研究的基础设施，并计划在西弗吉尼亚大学对研究企业进行重大扩展，以及支持西弗吉尼亚州IDEA卓越生物医学研究网络(WV-INBRE)。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal is submitted for a Becton-Dickinson (BD) FACSAria cell sorter to be housed in the Flow Cytometry Core Facility at West Virginia University. The Flow Cytometry Core Facility supports researchers from the basic sciences departments of the Health Sciences Center, the Mary Babb Randolph Cancer Center, and other regional campuses. The Facility was established 3 years ago as part of a Center for Biomedical Research Excellence grant from the National Center for Research Resources, and has experienced substantial growth in use and demand for additional services. Instrumentation currently includes a BD FACSCalibur and BD FACStarPlus cell sorter. While the FACScalibur is state of the art, the sorter is over 17 years old, is difficult to maintain due to its age, and has limited data acquisition and cell sorting capabilities. Acquisition of a BD FACSAria will provide >9 parameter cell analysis and high throughput cell sorting including sorting based on cell cycle using Hoechst dyes. Improved environmental control and biohazard containment will also facilitate sorting cells from infected animals and human cells, which will significantly enhance the Facility's capabilities. New instrumentation will benefit the HSC in several tangible ways including bolstering efforts to elevate the Mary Babb Randolph Cancer Center to an NCI-designated Clinical Cancer Center, improving infrastructure for ongoing research and a planned major expansion of the research enterprise at WVU, and supporting the West Virginia IDeA Network of Biomedical Research Excellence (WV-INBRE).