BECTON-DICKINSON FACSARIA FLOW CYTOMETER: LUNG CANCER, OVARIAN CANCER

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：本提案是针对Becton-Dickinson（BD）FACSAria细胞分选仪提交的，该细胞分选仪将安装在西弗吉尼亚大学的流式细胞术核心设施中。流式细胞术核心设施支持来自健康科学中心、玛丽巴布兰多夫癌症中心和其他地区校区的基础科学部门的研究人员。该设施成立于3年前，是国家研究资源中心生物医学研究卓越中心赠款的一部分，并且在使用和对额外服务的需求方面经历了大幅增长。仪器目前包括BD FACSCalibur和BD FACStarPlus细胞分选仪。虽然FACScalibur是最先进的，但分选机已经使用了17年以上，由于其年龄而难以维护，并且数据采集和细胞分选能力有限。获得BD FACSAria将提供>9个参数的细胞分析和高通量细胞分选，包括使用Hoechst染料基于细胞周期的分选。改善环境控制和生物危害控制也将有助于从受感染动物和人类细胞中分选细胞，这将大大提高该设施的能力。新的仪器将有利于HSC在几个有形的方式，包括加强努力，以提升玛丽巴布兰多夫癌症中心，以NCI指定的临床癌症中心，改善基础设施，为正在进行的研究和计划的重大扩展的研究企业在西弗吉尼亚大学，并支持生物医学研究卓越的西弗吉尼亚州IDEA网络（WV-INBRE）。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal is submitted for a Becton-Dickinson (BD) FACSAria cell sorter to be housed in the Flow Cytometry Core Facility at West Virginia University. The Flow Cytometry Core Facility supports researchers from the basic sciences departments of the Health Sciences Center, the Mary Babb Randolph Cancer Center, and other regional campuses. The Facility was established 3 years ago as part of a Center for Biomedical Research Excellence grant from the National Center for Research Resources, and has experienced substantial growth in use and demand for additional services. Instrumentation currently includes a BD FACSCalibur and BD FACStarPlus cell sorter. While the FACScalibur is state of the art, the sorter is over 17 years old, is difficult to maintain due to its age, and has limited data acquisition and cell sorting capabilities. Acquisition of a BD FACSAria will provide >9 parameter cell analysis and high throughput cell sorting including sorting based on cell cycle using Hoechst dyes. Improved environmental control and biohazard containment will also facilitate sorting cells from infected animals and human cells, which will significantly enhance the Facility's capabilities. New instrumentation will benefit the HSC in several tangible ways including bolstering efforts to elevate the Mary Babb Randolph Cancer Center to an NCI-designated Clinical Cancer Center, improving infrastructure for ongoing research and a planned major expansion of the research enterprise at WVU, and supporting the West Virginia IDeA Network of Biomedical Research Excellence (WV-INBRE).