BECTON-DICKINSON FACSARIA FLOW CYTOMETER: LUNG CANCER, OVARIAN CANCER

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本提案提交了一款Becton-Dickinson (BD) FACSAria细胞分选仪，该分选仪将被安置在西弗吉尼亚大学的流式细胞术核心设施中。流式细胞仪核心设备为来自健康科学中心、Mary Babb Randolph癌症中心和其他地区校区的基础科学系的研究人员提供支持。该基金是3年前作为国家研究资源中心的生物医学卓越研究中心拨款的一部分建立的，并且在使用和对额外服务的需求方面有了大幅增长。仪器目前包括BD FACSCalibur和BD FACStarPlus细胞分选器。虽然FACScalibur是最先进的，但该分选器已经使用了17年以上，由于其使用年限而难以维护，并且数据采集和细胞分选能力有限。收购BD FACSAria将提供bbb9参数细胞分析和高通量细胞分选，包括使用Hoechst染料进行基于细胞周期的分选。环境控制和生物危害控制的改善也将有助于从受感染的动物和人类细胞中分选细胞，这将大大提高该设施的能力。新的仪器将在几个切实的方面使HSC受益，包括支持将Mary Babb Randolph癌症中心提升为nci指定的临床癌症中心的努力，改善正在进行的研究的基础设施和西弗吉尼亚大学研究企业的计划主要扩张，并支持西弗吉尼亚IDeA生物医学研究卓越网络（WV-INBRE）。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal is submitted for a Becton-Dickinson (BD) FACSAria cell sorter to be housed in the Flow Cytometry Core Facility at West Virginia University. The Flow Cytometry Core Facility supports researchers from the basic sciences departments of the Health Sciences Center, the Mary Babb Randolph Cancer Center, and other regional campuses. The Facility was established 3 years ago as part of a Center for Biomedical Research Excellence grant from the National Center for Research Resources, and has experienced substantial growth in use and demand for additional services. Instrumentation currently includes a BD FACSCalibur and BD FACStarPlus cell sorter. While the FACScalibur is state of the art, the sorter is over 17 years old, is difficult to maintain due to its age, and has limited data acquisition and cell sorting capabilities. Acquisition of a BD FACSAria will provide >9 parameter cell analysis and high throughput cell sorting including sorting based on cell cycle using Hoechst dyes. Improved environmental control and biohazard containment will also facilitate sorting cells from infected animals and human cells, which will significantly enhance the Facility's capabilities. New instrumentation will benefit the HSC in several tangible ways including bolstering efforts to elevate the Mary Babb Randolph Cancer Center to an NCI-designated Clinical Cancer Center, improving infrastructure for ongoing research and a planned major expansion of the research enterprise at WVU, and supporting the West Virginia IDeA Network of Biomedical Research Excellence (WV-INBRE).