Special Becton Dickinson Fortessa Flow Cytometer

描述（由申请人提供）：西弗吉尼亚大学（WVU）医学院的流式细胞术核心设施（FCCF）是一个集中的机构共享研究资源设施，广泛服务于WVU和其他区域机构的研究社区，并按照机构共享研究设施管理的当代原则运作。FCCF的主要优先事项是支持NIH资助的生物医学研究项目，包括由NIGMS/NCRR的生物医学研究卓越中心（CoBRE）和生物医学研究卓越理念网络（INBRE）赠款资助的研究。FCCF的主要仪器包括一台2激光Becton Dickinson（BD）FACSCalibur分析流式细胞仪，这是由机构资金购买的，作为WVU对生物医学研究的承诺的一部分，自2002年以来一直在运行，以及一台3激光BD FACSAria细胞分选仪，这是在2006年由NIH共享仪器赠款购买的。当前提案描述了用LSRFortessa替换FACSCalibur的计划。此次升级将以三种切实的方式使研究界受益。它将：1）使研究人员能够将他们的一些分析工作从有能力的人身上转移出来。 但更昂贵的FACSAria细胞分选仪，从而提高整体效率并降低个体研究者的成本; 2）由于LSRFortessa提供的先进技术，提高了FCCF中生成的数据的质量;以及3）提高FCCF中分析服务的可用性，因为在过去的两年中，尽管FCCF科学家进行了勤奋的维护和仔细的监督，以及Becton Dickinson服务技术人员的详尽努力，但该公司仍有长期故障的历史。

DESCRIPTION (provided by applicant): The Flow Cytometry Core Facility (FCCF) at the West Virginia University (WVU) School of Medicine is a centralized institutional shared research resource facility that broadly serves the research community at WVU and other regional institutions, and is operated in accordance with contemporary principles in management of institutional shared research facilities. The main priority of the FCCF is to support NIH- funded projects in biomedical research, including research funded with grants from NIGMS/NCRR"s Center of Biomedical Research Excellence (CoBRE) and the Idea Network for Biomedical Research Excellence (INBRE) programs. Major instrumentation in the FCCF consists of a 2-laser Becton Dickinson (BD) FACSCalibur analytic flow cytometer, which was purchased with Institutional funds as part of WVU"s commitment to biomedical research and has been in operation since 2002, and a 3-laser BD FACSAria cell sorter, which was purchased in 2006 with an NIH Shared Instrumentation Grant. The current proposal describes a plan to replace the FACSCalibur with an LSRFortessa. This upgrade will benefit the research community in 3 tangible ways. It will: 1) enable researchers to shift some of their analytic work from the capable but more expensive FACSAria cell sorter, thereby improving overall efficiency and reducing costs to individual investigators; 2) improve the quality of the data being generated in the FCCF because of the advanced technology provided by the LSRFortessa; and 3) improve availability of analytic services in the FCCF because the FACSCalibur has, over the past 2 years, developed a history of chronic breakdowns despite diligent maintenance and careful oversight by FCCF scientists, as well as exhaustive efforts by Becton Dickinson service technicians.