Special Becton Dickinson Fortessa Flow Cytometer

描述（由申请人提供）：西弗吉尼亚大学（WVU）医学院的流式细胞仪核心设施（FCCF）是一个集中的机构共享研究资源设施，广泛服务于西弗吉尼亚大学和其他地区机构的研究界，并按照当代机构共享研究设施管理原则进行操作。FCCF的主要优先事项是支持NIH资助的生物医学研究项目，包括由NIGMS/NCRR的生物医学研究卓越中心（CoBRE）和生物医学研究卓越理念网络（INBRE）计划资助的研究。FCCF的主要仪器包括一台双激光Becton Dickinson (BD) FACSCalibur分析流式细胞仪，该仪器是作为WVU生物医学研究承诺的一部分使用机构基金购买的，自2002年以来一直在运行，以及一台三激光BD FACSAria细胞分选仪，该仪器是在2006年使用NIH共享仪器补助金购买的。目前的提案描述了用LSRFortessa取代FACSCalibur的计划。这一升级将以3种切实的方式使研究界受益。它将：1)使研究人员能够将他们的一些分析工作从能力转移

DESCRIPTION (provided by applicant): The Flow Cytometry Core Facility (FCCF) at the West Virginia University (WVU) School of Medicine is a centralized institutional shared research resource facility that broadly serves the research community at WVU and other regional institutions, and is operated in accordance with contemporary principles in management of institutional shared research facilities. The main priority of the FCCF is to support NIH- funded projects in biomedical research, including research funded with grants from NIGMS/NCRR"s Center of Biomedical Research Excellence (CoBRE) and the Idea Network for Biomedical Research Excellence (INBRE) programs. Major instrumentation in the FCCF consists of a 2-laser Becton Dickinson (BD) FACSCalibur analytic flow cytometer, which was purchased with Institutional funds as part of WVU"s commitment to biomedical research and has been in operation since 2002, and a 3-laser BD FACSAria cell sorter, which was purchased in 2006 with an NIH Shared Instrumentation Grant. The current proposal describes a plan to replace the FACSCalibur with an LSRFortessa. This upgrade will benefit the research community in 3 tangible ways. It will: 1) enable researchers to shift some of their analytic work from the capable but more expensive FACSAria cell sorter, thereby improving overall efficiency and reducing costs to individual investigators; 2) improve the quality of the data being generated in the FCCF because of the advanced technology provided by the LSRFortessa; and 3) improve availability of analytic services in the FCCF because the FACSCalibur has, over the past 2 years, developed a history of chronic breakdowns despite diligent maintenance and careful oversight by FCCF scientists, as well as exhaustive efforts by Becton Dickinson service technicians.