Special Becton Dickinson Fortessa Flow Cytometer

描述(由申请人提供)：西弗吉尼亚大学(WVU)医学院的流式细胞术核心设施(FCCF)是一个中央机构共享研究资源设施，广泛服务于西弗吉尼亚大学和其他地区性机构的研究社区，并按照当代机构共享研究设施的管理原则运作。FCCF的主要任务是支持NIH资助的生物医学研究项目，包括由NIGMS/NCRR S生物医学研究卓越中心和IDEA生物医学研究卓越计划资助的研究。FCCF的主要仪器包括一台2激光器Becton Dickinson(BD)FACSCalibur分析流式细胞仪，它是用机构资金购买的，是西弗吉尼亚大学S对生物医学研究的承诺的一部分，自2002年开始运行；以及一台3激光器BD FACSAria细胞分选器，它是在2006年与NIH共享仪器赠款购买的。目前的提案描述了一项用LSRFortessa取代FACSCalibur的计划。这一升级将从3个具体方面惠及研究界。它将：1)使研究人员能够将他们的一些分析工作从有能力的 但FACSAria细胞分选器更昂贵，从而提高了整体效率，并降低了单个调查人员的成本；2)由于LSRFortessa提供的先进技术，提高了FCCF中生成的数据的质量；以及3)提高了FCCF中分析服务的可用性，因为FACSCalibur在过去2年中发展了慢性故障的历史，尽管FCCF的科学家勤奋地维护和仔细的监督，以及Becton Dickinson服务技术人员的竭尽全力。

DESCRIPTION (provided by applicant): The Flow Cytometry Core Facility (FCCF) at the West Virginia University (WVU) School of Medicine is a centralized institutional shared research resource facility that broadly serves the research community at WVU and other regional institutions, and is operated in accordance with contemporary principles in management of institutional shared research facilities. The main priority of the FCCF is to support NIH- funded projects in biomedical research, including research funded with grants from NIGMS/NCRR"s Center of Biomedical Research Excellence (CoBRE) and the Idea Network for Biomedical Research Excellence (INBRE) programs. Major instrumentation in the FCCF consists of a 2-laser Becton Dickinson (BD) FACSCalibur analytic flow cytometer, which was purchased with Institutional funds as part of WVU"s commitment to biomedical research and has been in operation since 2002, and a 3-laser BD FACSAria cell sorter, which was purchased in 2006 with an NIH Shared Instrumentation Grant. The current proposal describes a plan to replace the FACSCalibur with an LSRFortessa. This upgrade will benefit the research community in 3 tangible ways. It will: 1) enable researchers to shift some of their analytic work from the capable but more expensive FACSAria cell sorter, thereby improving overall efficiency and reducing costs to individual investigators; 2) improve the quality of the data being generated in the FCCF because of the advanced technology provided by the LSRFortessa; and 3) improve availability of analytic services in the FCCF because the FACSCalibur has, over the past 2 years, developed a history of chronic breakdowns despite diligent maintenance and careful oversight by FCCF scientists, as well as exhaustive efforts by Becton Dickinson service technicians.