BECTON-DICKINSON FACSARIA FLOW CYTOMETER: INTESTINAL INFECTION

BECTON-DICKINSON FACSARIA 流式细胞仪：肠道感染

• 批准号: 7334982
• 负责人: Christopher F Cuff
• 金额: $ 8.51万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7334982
• 关键词: cell sorting; flow cytometry; infection; neoplasm /cancer

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal is submitted for a Becton-Dickinson (BD) FACSAria cell sorter to be housed in the Flow Cytometry Core Facility at West Virginia University. The Flow Cytometry Core Facility supports researchers from the basic sciences departments of the Health Sciences Center, the Mary Babb Randolph Cancer Center, and other regional campuses. The Facility was established 3 years ago as part of a Center for Biomedical Research Excellence grant from the National Center for Research Resources, and has experienced substantial growth in use and demand for additional services. Instrumentation currently includes a BD FACSCalibur and BD FACStarPlus cell sorter. While the FACScalibur is state of the art, the sorter is over 17 years old, is difficult to maintain due to its age, and has limited data acquisition and cell sorting capabilities. Acquisition of a BD FACSAria will provide >9 parameter cell analysis and high throughput cell sorting including sorting based on cell cycle using Hoechst dyes. Improved environmental control and biohazard containment will also facilitate sorting cells from infected animals and human cells, which will significantly enhance the Facility's capabilities. New instrumentation will benefit the HSC in several tangible ways including bolstering efforts to elevate the Mary Babb Randolph Cancer Center to an NCI-designated Clinical Cancer Center, improving infrastructure for ongoing research and a planned major expansion of the research enterprise at WVU, and supporting the West Virginia IDeA Network of Biomedical Research Excellence (WV-INBRE).

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本提案是针对安装在西弗吉尼亚大学流式细胞术核心设施中的 Becton-Dickinson (BD) FACSAria 细胞分选仪提交的。流式细胞术核心设施为健康科学中心、玛丽·巴布·伦道夫癌症中心和其他地区校园的基础科学部门的研究人员提供支持。该设施成立于三年前，是国家研究资源中心生物医学研究卓越中心拨款的一部分，并且在使用和对附加服务的需求方面经历了大幅增长。目前仪器包括 BD FACSCalibur 和 BD FACStarPlus 细胞分选仪。虽然 FACScalibur 是最先进的，但该分选机已有 17 年以上的历史，由于其老化而难以维护，并且数据采集和细胞分选能力有限。购买 BD FACSAria 将提供 >9 参数细胞分析和高通量细胞分选，包括使用 Hoechst 染料根据细胞周期进行分选。改进的环境控制和生物危害遏制也将有助于从受感染的动物和人类细胞中分选细胞，这将显着增强该设施的能力。新仪器将以多种切实的方式使 HSC 受益，包括加强将 Mary Babb Randolph 癌症中心提升为 NCI 指定的临床癌症中心、改善正在进行的研究的基础设施和计划对西弗吉尼亚大学研究企业进行重大扩展，以及支持西弗吉尼亚州 IDeA 生物医学研究卓越网络 (WV-INBRE)。