MICROARRAY SCANNER AND ACCESSORIES FOR MUSC

MUSC 微阵列扫描仪和配件

• 批准号: 7335042
• 负责人: WILLIAM SCOTT ARGRAVES
• 金额: $ 14.02万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335042
• 关键词: DNA; bioinformatics; diagnosis; gene expression; high throughput technology; microarray technology; phenotype; single nucleotide polymorphism; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): High throughput analysis of gene expression through the use of DNA microarray technology has become established as a powerful tool for biomedical research. Its applications are broad, ranging from sequencing and characterization of single nucleotide polymorphisms, to diagnosis of biological or pathogenic phenotypes, and to the transcriptomic profiling of biological samples. The Medical University of South Carolina (MUSC) DNA Microarray and Bioinformatics Facility was established in 2001 to provide investigators from MUSC and other South Carolina institutions with a comprehensive infrastructure comprised of faculty, support staff, state-of-the-art instrumentation and advanced computational/statistical analysis resources required to perform DNA microarray analysis. To date, this facility has been implemented in over 30 research projects, including those of 18 NIH-funded investigators in South Carolina. However, new advances in microarray technology, the demand for even higher levels of throughput screening, and the imminent obsolescence of our present microarray instrumentation necessitate the acquisition of new instrumentation in order to facilitate ongoing and future microarray experimentation at MUSC. This proposal will outline the current importance of microarray technology to MUSC investigators, the limitations of existing microarray instrumentation at MUSC, and the necessity for replacing this instrumentation as well as adding additional technical capabilities. Specifically, funds are requested for purchasing an Affymetrix GeneChip Scanner 3000 Upgrade and a Fluidics Station 450 Upgrade.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：通过使用 DNA 微阵列技术进行基因表达的高通量分析已成为生物医学研究的强大工具。其应用广泛，从单核苷酸多态性的测序和表征，到生物或致病表型的诊断，以及生物样品的转录组分析。南卡罗来纳医科大学 (MUSC) DNA 微阵列和生物信息学设施成立于 2001 年，旨在为 MUSC 和其他南卡罗来纳州机构的研究人员提供全面的基础设施，包括执行 DNA 微阵列分析所需的教师、支持人员、最先进的仪器和先进的计算/统计分析资源。迄今为止，该设施已在 30 多个研究项目中实施，其中包括南卡罗来纳州 18 名 NIH 资助的研究人员的项目。然而，微阵列技术的新进步、对更高水平通量筛选的需求以及我们现有的微阵列仪器即将过时，需要采购新仪器，以促进 MUSC 正在进行和未来的微阵列实验。该提案将概述微阵列技术当前对 MUSC 研究人员的重要性、MUSC 现有微阵列仪器的局限性以及更换该仪器以及增加额外技术能力的必要性。具体来说，需要资金来购买 Affymetrix GeneChip Scanner 3000 升级版和 Fluidics Station 450 升级版。