PROTEOGENOMICS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 蛋白质基因组学(核心C)的目的是为项目研究人员提供专家咨询、技术培训和最先进的仪器设备的资源，以便通过应用高通量基因组分析、定量聚合酶链式反应和基于蛋白质的(蛋白质组)方法来促进他们的研究工作。基因组部分允许研究人员使用几种基于微阵列的技术，包括全面的基因表达谱分析、单核苷酸多态和染色质免疫沉淀芯片方法；核心还为这些技术提供先进的计算/统计分析工具和培训。我们最近还通过增加RNA多重分析和激光捕获显微解剖组织来扩展我们的基因组学服务。蛋白质组学部分为研究人员提供技术援助和仪器资源，以使用传统的和尖端的基于蛋白质生物化学的技术，包括多重微珠阵列和表面等离子体技术来解决蛋白质功能问题。在过去的一年里，Cobre Core C继续为过去和现在的Cobre学员提供基因组和蛋白质组学服务(见附表1)，包括由Lipidology Cobre计划(RR107677，L.Obeid，PI)、口腔健康Cobre计划(RR017696)和SC INBRE计划(RR16461，L.Pirise-Creek，PI)资助的学员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of the Proteogenomics (Core C) is to provide project investigators with a resource for expert consultation, technical training, and state-of-the-art instrumentation to enable advancement of their research efforts through the application of high throughput genomic analysis, qPCR and protein based ('proteomic') approaches. The genomic component permits investigators to use several microarray-based technologies including comprehensive gene expression profiling, single nucleotide polymorphism, and chromatin immunoprecipitation-chip methods; the core also provides advanced computational/statistical analysis tools and training for these technologies. We have also recently expanded our genomics services by adding RNA multiplex analysis and laser capture microdissected tissue. The proteomics component provides investigators with technical assistance and instrumentation resources to use conventional and cutting edge protein biochemistry-based technologies including multiplex bead array and surface plasmon technology to address questions of protein function. During the last year COBRE Core C continued to provide genomics and proteomics services to both past and present COBRE trainees (see attached Table 1), including trainees funded by the Lipidomics COBRE program (RR107677, L. Obeid, PI) the Oral Health COBRE Program (RR017696) and the SC INBRE program (RR16461, L. Pirise-Creek, PI).