PROTEOGENOMICS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 蛋白质组学（核心C）的目的是为项目研究人员提供专家咨询，技术培训和最先进的仪器资源，以通过应用高通量基因组分析，qPCR和基于蛋白质的（“蛋白质组学”）方法来推进他们的研究工作。基因组部分允许研究人员使用几种基于微阵列的技术，包括全面的基因表达谱分析，单核苷酸多态性和染色质免疫沉淀芯片方法;核心还提供了先进的计算/统计分析工具和这些技术的培训。我们最近还通过增加RNA多重分析和激光捕获显微组织来扩大我们的基因组学服务。蛋白质组学部分为研究人员提供技术援助和仪器资源，以使用传统和尖端的蛋白质生物化学技术，包括多重珠阵列和表面等离子体技术，以解决蛋白质功能的问题。去年，COBRE Core C继续为过去和现在的COBRE学员提供基因组学和蛋白质组学服务（见附表1），包括由Lipidomics COBRE计划资助的学员（RR 107677，L. Obeid，PI）口腔健康COBRE计划（RR 017696）和SC INBRE计划（RR 16461，L. Pirise-Creek，PI）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of the Proteogenomics (Core C) is to provide project investigators with a resource for expert consultation, technical training, and state-of-the-art instrumentation to enable advancement of their research efforts through the application of high throughput genomic analysis, qPCR and protein based ('proteomic') approaches. The genomic component permits investigators to use several microarray-based technologies including comprehensive gene expression profiling, single nucleotide polymorphism, and chromatin immunoprecipitation-chip methods; the core also provides advanced computational/statistical analysis tools and training for these technologies. We have also recently expanded our genomics services by adding RNA multiplex analysis and laser capture microdissected tissue. The proteomics component provides investigators with technical assistance and instrumentation resources to use conventional and cutting edge protein biochemistry-based technologies including multiplex bead array and surface plasmon technology to address questions of protein function. During the last year COBRE Core C continued to provide genomics and proteomics services to both past and present COBRE trainees (see attached Table 1), including trainees funded by the Lipidomics COBRE program (RR107677, L. Obeid, PI) the Oral Health COBRE Program (RR017696) and the SC INBRE program (RR16461, L. Pirise-Creek, PI).