Flow Cytometry Shared Resource

流式细胞仪共享资源(FCSR) 项目摘要/摘要 流式细胞仪共享资源(FCSR)提供最先进的细胞分类和分析细胞术， 以及细胞学教育，向加州大学戴维斯综合癌症中心(UCDCCC)社区提供帮助。这个 FCSR是UCD唯一开放的流式细胞仪核心实验室，由细胞学专家组成，他们 随时可以帮助用户进行实验计划、仪器设备培训、操作员辅助电池 分类、数据分析、故障排除、手稿准备和审查以及高级数据分析，使用 流行的FlowJo流式细胞仪分析软件中的先进主成分分析工具 包裹。FCSR在三个地点设有设施，一个在萨克拉门托的加州大学戴维斯分校健康园区，两个在 戴维斯校区，提供各种各样的细胞检测设备。萨克拉门托工厂是BD的所在地 Fortessa、BD FACS Canto I和Stratedigm S1000EX分析细胞仪，以及BD流入和Aria II细胞 分拣员。Davis工厂配备了新的Cytek Aurora光谱细胞分析仪Becton Dickinson LSR-II， FACScan和Beckman Coulter Cytoflex细胞分析仪，以及Astrios和6色BD FACSCanto II细胞分选仪， 以及用于单细胞基因表达分析的Fluidigm BioMark系统。FCSR支持广泛的 UCDCCC的基础研究项目旨在阐明癌症病因、转移、 进展和治疗。BRSR的监督和管理由主任Barbara L. 沙克利特博士，免疫学家和病毒学家，有20多年使用流式细胞术的经验 她的研究方法；技术总监，布里奇特·麦克劳克林，MS，公认的校园领导者 在细胞检测技术、仪器和教育方面有20年的经验和特殊的专业知识 在多色流式细胞术和分析中。FCSR人员定期咨询UCDCCC成员以提供帮助 开发新的细胞分析和分类方法。FCSR提供一年一度的综合课程 在流式细胞术方面，包括流式细胞术的基础知识、高级课题和实践培训。这个 UCDCCC领导层为从事癌症相关研究的学生提供奖学金。具体的 FCSR的目标是：1)提供最先进的细胞分类和分析细胞术。2)建议用户 关于适当的实验设计和数据分析。3)教育用户有关常规和切割的知识- 边缘细胞术的应用。4)促进使用这些技术的研究人员之间的互动。5)刺激 开发癌症研究的新方法。

FLOW CYTOMETRY SHARED RESOURCE (FCSR) PROJECT SUMMARY/ABSTRACT The Flow Cytometry Shared Resource (FCSR) provides state-of-the-art cell sorting and analytical cytometry, as well as cytometry education, to the UC Davis Comprehensive Cancer Center (UCDCCC) community. The FCSR is the only openly available flow cytometry core laboratory at UCD staffed by cytometry experts who are readily available to assist users with experiment planning, instrumentation training, operator-assisted cell sorting, data analysis, troubleshooting, manuscript preparation and review, and advanced data analysis, using the advanced principal component analysis tools in the popular FlowJo flow cytometry analysis software package. FCSR has facilities at three sites, one at the UC Davis Health campus in Sacramento, and two on the Davis campus, offering a wide variety of cytometry equipment. The Sacramento facility houses the BD Fortessa, BD FACS Canto I, and Stratedigm S1000EX analytic cytometers, and the BD inFlux and Aria II cell sorters. The Davis facilities house a new Cytek Aurora spectral cell analyzer, Becton Dickinson LSR-II, FACScan, and Beckman Coulter Cytoflex cell analyzers, and Astrios and 6-color BD FACSCanto II cell sorters, and a Fluidigm BioMark system for gene expression analysis in single cells. The FCSR supports a wide range of basic research projects at UCDCCC aimed at elucidating mechanisms in cancer etiology, metastasis, progression, and treatment. Oversight and management of the BRSR is provided by the Director, Barbara L. Shacklett, PhD an immunologist and virologist with over 20 years of experience using flow cytometry approaches in her research; and the technical director, Bridget McLaughlin, MS, a recognized campus leader in with 20 years of experience in cytometry techniques, instrumentation and education and particular expertise in multicolor flow cytometry and analysis. FCSR personnel regularly consult with UCDCCC members to help develop new approaches for cellular analysis and sorting. The FCSR offers an annual comprehensive course in Flow Cytometry that includes flow cytometry fundamentals, advanced topics, and hands on training. The UCDCCC leadership sponsors scholarships for students conducting cancer-related research. The Specific Aims for the FCSR are to: 1) Provide state-of-the-art cell sorting and analytical cytometry. 2) Advise users about appropriate experimental design and data analysis. 3) Educate users about routine as well as cutting- edge cytometry applications. 4) Facilitate interactions among researchers using these techniques. 5) Stimulate development of new approaches for cancer research.