Stem Cells for Corneal Engineering
角膜工程干细胞
基本信息
- 批准号:7221869
- 负责人:
- 金额:$ 30.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-04-15 至 2011-03-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAdultAgingArchitectureBiologicalBiological MarkersBiomedical EngineeringBiophysicsBioprosthesis deviceBlindnessCell TherapyCellsCharacteristicsCicatrixCollagenCollagen FibrilConditionConnective TissueCorneaCorneal InjuryCorneal StromaDepositionEngineeringEpithelialExhibitsExtracellular MatrixFibroblastsGenerationsGoalsHealedHumanImplantIn VitroIndividualInjection of therapeutic agentKnockout MiceLaboratoriesLamellar KeratoplastyLeftLocationMolecularMusMyofibroblastNatural regenerationNeural CrestOperative Surgical ProceduresPathologicPathologyPhenotypePopulationPropertyProteoglycanProtocols documentationPublic HealthResearch PersonnelRoleSerial PassageStem cellsStressStructureTechnologyTestingTherapeutic InterventionTimeTissuesTransforming Growth Factor betaTransplantationVisionadult stem cellbasecell watercorneal scarfallshealinghuman stem cellsin vivolimballumicanmouse modelnovelprogramsremediationrepairedresearch studyresponsescaffoldtransdifferentiation
项目摘要
DESCRIPTION: Aging of the population and popularity of refractive surgery threaten adequacy of the cornea supply used for transplantation in the USA. World-wide, cornea donations already fall short, leaving 6-8 million individuals suffering corneal blindness. Engineered corneas, grown in the laboratory and available on demand, will ultimately help alleviate this public health problem. The long-term goal of this project is to develop corneal equivalents for transplantation using cultured human stem cells. Initially the project will focus on the corneal stroma, the connective tissue that makes up 90% of the corneal mass. We have recently discovered a population of cells from human corneal stroma with properties of adult stem cells. Unlike keratocytes, the human corneal stromal stem cells (hCSSC) can be passaged numerous times in culture but still retain the ability to become keratocytes in vivo and in vitro. We hypothesize that the human corneal stromal stem cells participate in response to corneal injury and can regenerate transparent stromal tissue both in vivo and in vitro. This hypothesis will be tested (1) by demonstrating the influx of hCSSC into pathological human corneas and by documenting the fate of hCSSC introduced into normal and healing mouse corneas in vivo. (2) Lumican null mice which develop opacity similar to corneal scars and corneas scarred by injection of transforming growth factor beta will be treated with hCSSC to investigate observed ability of these cells to restore corneal transparency. (3) Tissue equivalents produced in vitro by hCSSC will be implanted into mouse cornea to investigate the molecular and structural requirements for transparency in stromal tissue. Successful accomplishment of these aims will elucidate biological roles of the newly discovered adult stromal stem cells and provide novel information about how the stromal ultrastructure and corneal transparency are maintained in vivo. The experiments will also develop essential technologies for cell-based therapy for corneal scars and for fabrication of bioengineered tissue which could be used directly for lamellar
keratoplasty or serve as the basis of a fully bioengineered cornea.
描述:人口老龄化和屈光手术的普及威胁着美国用于移植的角膜供应的充足性。在世界范围内,角膜捐赠已经不足,导致600万至800万人患有角膜失明。在实验室中培养并按需提供的工程角膜最终将有助于缓解这一公共卫生问题。该项目的长期目标是利用培养的人类干细胞开发用于移植的角膜等效物。最初,该项目将重点关注角膜基质,即构成90%角膜质量的结缔组织。我们最近从人角膜基质中发现了一群具有成体干细胞特性的细胞。与角膜基质细胞不同,人角膜基质干细胞(hCSSC)可以在培养物中传代多次,但仍保留在体内和体外成为角膜基质细胞的能力。我们假设人角膜基质干细胞参与了角膜损伤的反应,并能在体内和体外再生透明的基质组织。将通过证明hCSSC流入病理性人角膜和通过记录体内引入正常和愈合小鼠角膜的hCSSC的命运来测试该假设(1)。 (2)将用hCSSC处理产生类似于角膜瘢痕和通过注射转化生长因子β而瘢痕化的角膜的不透明的Lumican无效小鼠,以研究观察到的这些细胞恢复角膜透明度的能力。(3)将由hCSSC体外产生的组织等同物植入小鼠角膜中,以研究基质组织中透明度的分子和结构要求。 这些目标的成功实现将阐明新发现的成体基质干细胞的生物学作用,并提供有关基质超微结构和角膜透明度如何在体内维持的新信息。这些实验还将开发基于细胞的角膜瘢痕治疗和生物工程组织制造的基本技术,这些生物工程组织可直接用于层状角膜。
角膜移植术或作为完全生物工程角膜的基础。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(2)
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JAMES L FUNDERBURGH其他文献
JAMES L FUNDERBURGH的其他文献
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{{ truncateString('JAMES L FUNDERBURGH', 18)}}的其他基金
A Pluripotent Stem Cell Reagent for Corneal Regeneration
用于角膜再生的多能干细胞试剂
- 批准号:
8626779 - 财政年份:2013
- 资助金额:
$ 30.9万 - 项目类别:
A Pluripotent Stem Cell Reagent for Corneal Regeneration
用于角膜再生的多能干细胞试剂
- 批准号:
8776715 - 财政年份:2013
- 资助金额:
$ 30.9万 - 项目类别:
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