Genetic Dissection Of Stem Cell Function

成体和胚胎干细胞具有巨大的治疗潜力。造血干细胞已经被最深入的研究，并广泛用于治疗环境，但我们只有一个补丁知识的基因所需的增殖和生存。我将在小鼠中使用经典的遗传筛选来识别调节干细胞行为的基因。我将分析两个现有的突变小鼠品系与数量减少的造血干细胞，并执行一个新的遗传筛选利用小鼠的缺陷，在自我更新的成人造血和神经干细胞，找到小鼠与恢复干细胞隔室。

Adult and embryonic stem cells have enormous therapeutic potential. Haemopoietic stem cells have been the most intensely studied and widely used in a therapeutic setting, yet we have only a patchy knowledge of the genes required for their proliferation and survival. I will use classical genetic screens in the mouse to identify genes that regulate stem cell behaviour. I will analyse two existing mutant mouse strains with reduced numbers of haemopoietic stem cells, and execute a novel genetic screen utilising mice with a defect in the self-renewal of adult haemopoietic and neural stem cells, to find mice with a recovered stem cell compartment.