Low Cost Conductimetric Biosensor for Bacterial Meningitis in Cerebrospinal Fluid

用于脑脊液中细菌性脑膜炎的低成本电导生物传感器

基本信息

  • 批准号:
    7271816
  • 负责人:
  • 金额:
    $ 14.27万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-06-15 至 2009-05-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): There is an urgent need for rapid diagnostics that are highly sensitive, specific, inexpensive, easy to use, and located in primary care settings to identify bacteria and viruses in cerebrospinal fluid (CSF). The long-term goal of this project is to develop a syndrome-based, multi-target immuno-biosensor for diagnosis of meningitis that can be used as a "first response" device in hospitals and clinics. The biosensor will target multiple meningitis agents in a single test to identify the etiology in symptomatic patients. The proposed sensor, invented at Michigan State University, uses a conductimetric detection technique to produce qualitative results in 6 to 10 minutes with a lower detection limit of 79 cells/ml for bacteria and 103 viral particles. Rapid BioSense LLC holds an exclusive license for the patent (under review). The overall goal of the project is to transfer this previously demonstrated technology from a University laboratory experiment into a commercially viable product. The overall aim of the Phase I program is to demonstrate the feasibility of the technology as a single pathogen assay for meningitis diagnostics using Neisseria meningitidis in porcine CSF samples and Trans-Isolate medium (a CSF transport medium). The Phase I program is focused on three specific aims: Aim 1: Fabrication of a single-target biosensor for N. meningitidis Using methods from preliminary studies, single-target biosensors will be fabricated for detection of N. meningitidis grown in broth media. Aim 2: Biosensor testing for N. meningitidis in CSF samples Protocols will be developed for testing the biosensor with N. meningitidis in porcine CSF samples (including some blood-tainted CSF samples). Feasibility of the biosensor for human diagnostics will be demonstrated using animal model, and performance of the sensor will be characterized. Combined sample preparation and test time is aimed at less than 30 minutes. Aim 3: Biosensor testing for N. meningitidis in Trans-Isolate (T-I) medium Feasibility of the biosensor for use with the T-I transport medium will be demonstrated, and performance of the sensor characterized. The Phase II program will include development of a general meningitis test and a multi-array device for detection of multiple pathogens that cause meningitis, as well as improved sensor design, conductive ink formulation, and application technique for the capture pad electrodes. Following Phase II, clinical trials will be conducted, and FDA approvals for the diagnostic device will then be sought. The proposed biosensor is inexpensive and well-suited to the task of rapidly detecting pathogens causing bacterial meningitis. This will help healthcare providers quickly identify the potential cause of meningitis (bacterial or viral), collaborate with epidemiologists to determine the source of an epidemic and minimize its effects, achieve faster prognosis in patients, and aid targeted treatment of the disease. This is important in preventing increase in resistance of pathogens to antibiotics resulting from indiscriminate use of antibiotics, as well as reducing health costs and man-hours lost associated with misdiagnosed illnesses, unnecessary hospitalizations and tests performed. Commercialization of the conductimetric sensor technology will enable improvement in the overall quality of human life, not only in the US but around the world.
描述(由申请人提供):迫切需要高度敏感、特异、廉价、易于使用且位于初级保健环境中的快速诊断方法,以识别脑脊液(CSF)中的细菌和病毒。该项目的长期目标是开发一种基于症状的多靶点免疫生物传感器,用于诊断脑膜炎,可用作医院和诊所的"第一反应"设备。生物传感器将在一次测试中针对多种脑膜炎病原体,以确定有症状患者的病因。该传感器由密歇根州立大学发明,采用电导检测技术,在6至10分钟内产生定性结果,细菌和103病毒颗粒的检测下限为79个细胞/ml。Rapid BioSense LLC持有该专利的独家许可(正在审查中)。该项目的总体目标是将先前演示的技术从大学实验室实验转化为商业上可行的产品。I期项目的总体目标是证明该技术作为使用猪CSF样本和Trans-Isolate培养基(CSF转运培养基)中的脑膜炎奈瑟菌进行脑膜炎诊断的单一病原体检测的可行性。 第一阶段计划的重点是三个具体目标: 目的1:制备单靶点N.脑膜 利用初步研究的方法,单靶生物传感器将被制作用于检测N。在肉汤培养基中生长的脑膜炎病毒。 目的2:N. CSF样本中的脑膜炎 将开发用于测试生物传感器的协议与N。猪CSF样本(包括一些血液污染的CSF样本)中的脑膜炎。将使用动物模型证明生物传感器用于人类诊断的可行性,并表征传感器的性能。样品制备和测试的总时间目标是少于30分钟。 目的3:N.在反式分离物(T-I)培养基中的脑膜炎 将证明与T-I转运介质一起使用的生物传感器的可行性,并表征传感器的性能。 第二阶段计划将包括开发一般脑膜炎测试和用于检测导致脑膜炎的多种病原体的多阵列设备,以及改进的传感器设计,导电油墨配方和捕获垫电极的应用技术。在第二阶段之后,将进行临床试验,然后将寻求FDA对诊断设备的批准。 所提出的生物传感器是廉价的,非常适合的任务,快速检测病原体引起细菌性脑膜炎。这将有助于医疗保健提供者快速确定脑膜炎(细菌或病毒)的潜在原因,与流行病学家合作确定流行病的来源并最大限度地减少其影响,实现更快的患者预后,并帮助有针对性地治疗疾病。这对于防止由于滥用抗生素而导致病原体对抗生素的耐药性增加,以及减少与误诊疾病、不必要的住院治疗和进行的检查有关的保健费用和工时损失,都很重要。电导传感器技术的商业化将不仅在美国而且在世界各地提高人类生活的整体质量。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Finny Mathew其他文献

Finny Mathew的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Finny Mathew', 18)}}的其他基金

Rapid, low cost, point-of-care diagnostic device for Group B streptococcus
B 族链球菌快速、低成本的现场诊断设备
  • 批准号:
    7671540
  • 财政年份:
    2009
  • 资助金额:
    $ 14.27万
  • 项目类别:

相似海外基金

Can antibiotics disrupt biogeochemical nitrogen cycling in the coastal ocean?
抗生素会破坏沿海海洋的生物地球化学氮循环吗?
  • 批准号:
    2902098
  • 财政年份:
    2024
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Studentship
Metallo-Peptides: Arming Cyclic Peptide Antibiotics with New Weapons to Combat Antimicrobial Resistance
金属肽:用新武器武装环肽抗生素以对抗抗菌素耐药性
  • 批准号:
    EP/Z533026/1
  • 财政年份:
    2024
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Research Grant
The role of RNA repair in bacterial responses to translation-inhibiting antibiotics
RNA修复在细菌对翻译抑制抗生素的反应中的作用
  • 批准号:
    BB/Y004035/1
  • 财政年份:
    2024
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Research Grant
Towards the sustainable discovery and development of new antibiotics
迈向新抗生素的可持续发现和开发
  • 批准号:
    FT230100468
  • 财政年份:
    2024
  • 资助金额:
    $ 14.27万
  • 项目类别:
    ARC Future Fellowships
DYNBIOTICS - Understanding the dynamics of antibiotics transport in individual bacteria
DYNBIOTICS - 了解抗生素在单个细菌中转运的动态
  • 批准号:
    EP/Y023528/1
  • 财政年份:
    2024
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Research Grant
Engineering Streptomyces bacteria for the sustainable manufacture of antibiotics
工程化链霉菌用于抗生素的可持续生产
  • 批准号:
    BB/Y007611/1
  • 财政年份:
    2024
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Research Grant
The disulfide bond as a chemical tool in cyclic peptide antibiotics: engineering disulfide polymyxins and murepavadin
二硫键作为环肽抗生素的化学工具:工程化二硫多粘菌素和 murepavadin
  • 批准号:
    MR/Y033809/1
  • 财政年份:
    2024
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Research Grant
Role of phenotypic heterogeneity in mycobacterial persistence to antibiotics: Prospects for more effective treatment regimens
表型异质性在分枝杆菌对抗生素持久性中的作用:更有效治疗方案的前景
  • 批准号:
    494853
  • 财政年份:
    2023
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Operating Grants
Imbalance between cell biomass production and envelope biosynthesis underpins the bactericidal activity of cell wall -targeting antibiotics
细胞生物量产生和包膜生物合成之间的不平衡是细胞壁靶向抗生素杀菌活性的基础
  • 批准号:
    2884862
  • 财政年份:
    2023
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Studentship
Narrow spectrum antibiotics for the prevention and treatment of soft-rot plant disease
防治植物软腐病的窄谱抗生素
  • 批准号:
    2904356
  • 财政年份:
    2023
  • 资助金额:
    $ 14.27万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了