Development of Retinal Receptive Fields

视网膜感受野的发育

• 批准号: 7105502
• 负责人: NORBERTO M GRZYWACZ
• 金额: $ 35.43万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7105502
• 关键词: Chelonia; brain electrical activity; calcium indicator; cell growth regulation; computer simulation; dark adaptation; dendrites; developmental neurobiology; electrophysiology; fluorescence microscopy; imaging /visualization /scanning; mathematical model; neostigmine; neural information processing; neural plasticity; neurons; retinal ganglion; synapses; visual fields; visual pathways; visual phototransduction; visual stimulus

DESCRIPTION (provided by applicant): An important property of visual neurons is their receptive-field size. One long-term goal of the proposed research is to understand the developmental mechanisms controlling the growth of retinal receptive fields. We recently established that one such mechanism depends on the spontaneous waves of activity that sweep developing retinas. Four hypotheses for this mechanism involve dendritic-tree growth, optimization of receptive-field size, critical period for growth, and inter-cell competition. We will test these hypotheses in the turtle retina with four aims: 1) We will use intracellular recordings and dendritic staining to test whether spontaneous waves cause receptive fields of ganglion cells to grow by enlarging their dendritic trees. 2) Turtles will be reared in abnormal light environments to test through extracellular recordings whether receptive fields grow to a size optimized to average out noise, while not over-smoothing the image. 3) Ca2+ fluorescence will help us map the waves and ask how light affects them. 4) We will test whether receptive-field growth stops because of a critical period or of an inter-cell competition by increasing the intensity of dark-reared waves though the retinal implantation of drug-laced Elvax. This study could help understand several developmental pathologies affecting ganglion cells. These pathologies include retinopathy of prematurity, retinal degeneration, refsum disease, Leber hereditary optic neuropathy, optic-nerve hypoplasia, and drusen of the optic disk. Besides understanding pathologies, unraveling ganglion-cell plasticity may help with a promising technique for one of their cures, namely, retinal transplantation. In such a transplantation, cells must "self-organize" as during development.

描述（由申请人提供）：视觉神经元的一个重要特性是它们的感受野大小。该研究的长期目标之一是了解控制视网膜感受野生长的发育机制。我们最近发现，这样一种机制依赖于扫过正在发育的视网膜的自发活动波。该机制的四个假设涉及树突树的生长、感受野大小的优化、生长的关键期和细胞间竞争。我们将在乌龟视网膜上测试这些假设，有四个目标： 1）我们将使用细胞内记录和树突染色来测试自发波是否通过扩大树突树来导致神经节细胞的感受野生长。 2) 海龟将在异常光照环境中饲养，通过细胞外记录来测试感受野是否增长到优化的大小以平均噪声，同时不会过度平滑图像。 3) Ca2+ 荧光将帮助我们绘制波图并了解光如何影响它们。 4）我们将通过在视网膜植入药物Elvax来增加暗养波的强度来测试感受野生长是否由于关键时期或细胞间竞争而停止。 这项研究可以帮助了解影响神经节细胞的几种发育病理学。这些病理包括早产儿视网膜病、视网膜变性、视眼病、莱伯遗传性视神经病、视神经发育不全和视盘玻璃膜疣。除了了解病理学之外，解开神经节细胞可塑性可能有助于开发一种有前途的技术来治疗它们，即视网膜移植。在这样的移植中，细胞必须像在发育过程中一样“自组织”。

项目成果

Rearrangement of the cone mosaic in the retina of the rat model of retinitis pigmentosa.

• DOI: 10.1002/cne.22800
• 发表时间: 2012-03-01
• 期刊: JOURNAL OF COMPARATIVE NEUROLOGY
• 影响因子: 2.5
• 作者: Ji, Yerina;Zhu, Colleen L.;Grzywacz, Norberto M.;Lee, Eun-Jin
• 通讯作者: Lee, Eun-Jin

Morphological changes of short-wavelength cones in the developing S334ter-3 transgenic rat.

• DOI: 10.1016/j.brainres.2010.01.051
• 发表时间: 2010-03-19
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Hombrebueno, Jose R.;Tsai, Melody M.;Kim, Hong-Lim;De Juan, Joaquin;Grzywacz, Norberto M.;Lee, Eun-Jin
• 通讯作者: Lee, Eun-Jin

Role of Muller cells in cone mosaic rearrangement in a rat model of retinitis pigmentosa.

Muller 细胞在视网膜色素变性大鼠模型中锥体镶嵌重排中的作用。

• DOI: 10.1002/glia.21183
• 发表时间: 2011
• 期刊: Glia
• 影响因子: 6.2
• 作者: Lee,Eun-Jin;Ji,Yerina;Zhu,ColleenL;Grzywacz,NorbertoM
• 通讯作者: Grzywacz,NorbertoM

Spatiotemporal pattern of rod degeneration in the S334ter-line-3 rat model of retinitis pigmentosa.

S334ter-line-3 大鼠色素性视网膜炎模型中视杆细胞退化的时空模式。

• DOI: 10.1007/s00441-012-1522-5
• 发表时间: 2013
• 期刊: Cell and tissue research
• 影响因子: 3.6
• 作者: Zhu,ColleenL;Ji,Yerina;Lee,Eun-Jin;Grzywacz,NorbertoM
• 通讯作者: Grzywacz,NorbertoM

The effect of TIMP-1 on the cone mosaic in the retina of the rat model of retinitis pigmentosa.

TIMP-1对色素性视网膜炎大鼠模型视网膜锥体镶嵌的影响。

• DOI: 10.1167/iovs.14-15398
• 发表时间: 2014
• 期刊: Investigative ophthalmology & visual science
• 影响因子: 4.4
• 作者: Ji,Yerina;Yu,Wan-Qing;Eom,YunSung;Bruce,Farouk;Craft,CherylMae;Grzywacz,NorbertoM;Lee,Eun-Jin
• 通讯作者: Lee,Eun-Jin