Optical detection of neural activity with phase sensitive interferometry

使用相敏干涉测量法光学检测神经活动

• 批准号: 7243818
• 负责人: Boris Hyle Park
• 金额: $ 9万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7243818
• 关键词: Action Potentials; Address; Animal Model; Animals; Architecture; Award; Behavior; Biological Neural Networks; Brain; Chemicals; Clinical; Complement; Computer information processing; Detection; Development; Disease; Dyes; Electrodes; Electrophysiology (science); Evaluation; Eye; Fiber; Foundations; Funding; Future; Glaucoma; Goals; Histology; Image; In Vitro; Individual; Instruction; Interferometry; Invasive; Laboratories; Lateral; Lead; Light; Limulus polyphemus; Mentors; Methods; Nerve; Neurons; Neurosciences; Neurosciences Research; Optic Nerve; Optical Coherence Tomography; Optical Methods; Optics; Pattern; Phase; Physics; Physiological; Process; Range; Research; Research Personnel; Research Project Grants; Research Proposals; Resolution; Retina; Structure; Supervision; Swelling; System; Techniques; Technology; Time; Tissues; Training; Translating; United States National Institutes of Health; Visual system structure; Work; Xiphosura; base; bioimaging; career; clinical application; compound eye; experience; in vivo; insight; medical schools; nanoscale; novel; optical imaging; programs; receptor; relating to nervous system; research study; success; visual information; voltage

DESCRIPTION (provided by applicant): This K99/R00 proposal on the development of optical methods for imaging transient structural changes associated with neural activity integrates rigorous physics, biomedical, and neuroscience research, close career mentoring, and tailored coursework to establish Dr. B. Hyle Park as an independent investigator. Having completed his graduate research in physics, Dr. Park is now continuing his research at the Wellman Center for Photomedicine as a research fellow and is making the transition to research independence. He has focused on biomedical imaging, particularly in the development and clinical application of polarization- sensitive optical coherence tomography. Dr. Park now seeks to complement this grounding in biomedical optics with mentored research in the application of these optical techniques to neuroscience. His research proposal addresses the need for non-contact detection of neural structure and activity. Current techniques for detection of action potential propagation involve direct contact with electrodes or introduction of chemical markers, such as Ca++ dyes. In this K99/R00, he focuses on optical detection of transient structural changes that accompany spike propagation in neurons using phase-sensitive interferometry. Successful completion of the proposed research will enable optical detection of the structure and activity of nerves and neuronal ensembles in vitro, and lay the groundwork for in vivo application as well as providing the preliminary results necessary for a successful R01 application. This work not only has potential application as a complement to electrophysiology, but would be of use in clinically important regions, such as the retina, that are largely inaccessible with current techniques. Co-mentor Dr. Johannes F. de Boer has an established track record in the development of optical imaging methods and has successfully translated emerging research to ophthalmological and clinical use. Co-mentor Dr. Christopher L. Passaglia has experienced similar success in the study of how visual information is represented in the eye's input to the brain and how that changes as diseases, like glaucoma, inflict damage upon the retina. In addition to their supervision, Dr. Park will have access to coursework in neuroscience, and the ethical conduct of research at Harvard Medical School. The opportunities provided by this career award will allow Dr. Park to combine expertise in physics and biomedical optics with neuroscience, substantially adding to his capabilities as an independent researcher.

描述（由申请人提供）： 这个K99/R 00关于成像与神经活动相关的瞬态结构变化的光学方法的发展提案整合了严格的物理学，生物医学和神经科学研究，密切的职业指导和量身定制的课程，以建立B博士。Hyle Park作为独立调查员。在完成了他的物理学研究生研究后，Park博士现在作为研究员继续在Wellman光医学中心进行研究，并正在向独立研究过渡。他专注于生物医学成像，特别是偏振敏感光学相干断层扫描的开发和临床应用。Park博士现在寻求通过将这些光学技术应用于神经科学的指导研究来补充生物医学光学的基础。他的研究计划解决了非接触式检测神经结构和活动的需求。目前用于检测动作电位传播的技术涉及与电极直接接触或引入化学标记物，例如Ca++染料。在这个K99/R 00中，他专注于使用相敏干涉测量法对伴随神经元中尖峰传播的瞬态结构变化进行光学检测。拟议研究的成功完成将使体外神经和神经元集合的结构和活动的光学检测成为可能，并为体内应用奠定基础，并为R 01的成功应用提供必要的初步结果。这项工作不仅有潜在的应用作为电生理学的补充，但将在临床上重要的区域，如视网膜，这是目前的技术在很大程度上无法访问的使用。共同导师Johannes F. de Boer在光学成像方法的开发方面有着良好的记录，并成功地将新兴研究转化为眼科和临床应用。共同导师Christopher L.帕萨利亚在研究视觉信息如何在眼睛输入到大脑中的过程中表现出来，以及随着青光眼等疾病对视网膜造成损害而发生的变化方面也取得了类似的成功。除了他们的监督，朴博士将有机会在神经科学课程，并在哈佛医学院的研究道德行为。该职业奖提供的机会将使Park博士能够将物理学和生物医学光学方面的联合收割机专业知识与神经科学相结合，大大增强了他作为独立研究人员的能力。