Optical Detection of Neural Activity With Phase Sensitive Interferometry

使用相敏干涉测量法光学检测神经活动

• 批准号: 7714795
• 负责人: Boris Hyle Park
• 金额: $ 24.41万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714795
• 关键词: Action Potentials; Address; Animals; Area; Award; Behavior; Biological Neural Networks; Biomedical Engineering; California; Cell Count; Cells; Chemicals; Collaborations; Complement; Contrast Media; Detection; Development; Dyes; Educational process of instructing; Electrodes; Electrophysiology (science); Eye; Faculty; Fiber; Foundations; Funding; Future; Goals; Hand; Histocompatibility Testing; Image; In Vitro; Individual; Interferometry; Lateral; Lead; Light; Limulus; Limulus polyphemus; Magnetic Resonance Imaging; Mentors; Methods; Nerve; Neurons; Neurosciences; Neurosciences Research; Optic Nerve; Optical Coherence Tomography; Optical Methods; Optics; Phase; Physics; Physiological; Positioning Attribute; Process; Rattus; Research; Research Personnel; Research Project Grants; Research Proposals; Research Training; Resolution; Retina; Structure; Supervision; Swelling; Techniques; Technology; The Sun; Thick; Time; Training; Universities; Visual system structure; Work; Xiphosura; base; bioimaging; clinical application; compound eye; experience; in vitro activity; in vivo; minimally invasive; myelination; nanoscale; novel; optical imaging; receptor; relating to nervous system; sciatic nerve

This ROO transition application on the development of optical methods for imaging transient structural changes associated with neural activity integrates rigorous physics, biomedical, and neuroscience research to further the research goals of the original K99/R00 proposal. The mentored (K99) phase has allowed Dr. B. Hyle Park a successful transition to an independent research positioii in the Bioengineering department at the University of California, Riverside. The parameters of the position provide for start-up funding, space, and support that are comparable or exceed that of other recently hired faculty. Furthermore^ it also provides for at least 75% protected research time with a generous amount of teaching relief. The independent (ROO) phase will further Dr. Park's development as an independent researcher and facilitate successful application for an independent research grant (ROl). Having completed his graduate research in physics with a strong focus on biomedical imaging, particularly in the development and clinical application of polarization-sensitive optical coherence tomo^phy, Dr. Park has complemented this grounding in biomedical optics with coursework in and hands-on laboratoiy experience with current neuroscience techniques. His research proposal addresses the need for non-contact detection of neural structure and activity. Current techniques for detection of action potential propagation involve direct contact with electrodes or introduction of chemical markers, such as Ca^^ dyes. The focus of this K99/R00 is optical detection of transient structural changes that accompany spike propagation in neurons using a phasesensitive interferometric technique known as spectral-domain optical coherence tomography (SD-OCT). An important first step in optically detecting activity in nerves is the ability to identify them in SD-OCT images. During the mentored (K99) phase, it was shown that unmyelinated limulus lateral compound optic nerve and myelinated rat sciatic nerve can be idientifled fiom surrounding other tissue types in structural images, providing valuable groundwork for future in vivo application of optical detection of neural activity as it allows for differentiation of myelinated and non-myelinated neurons versus other tissue types within acquired images. These results also demonstrate that quantitative analysis of optical images of nerve structure is capable of non-destructive assessment of the degree of nerve myelination. A correlation between electrically recorded action potential propagation with optical detection of associated axonal thickness changes was established, and such detection is possible for single impulses without requiring averaging of results from repeated trials. The independent phase ofthis propos^ aims to further develop this optical technique such that it can be applied to detailed studies of the interaction between individual cells in and behavior of functional neural networks. Successful completion of the proposed research will enable optical detection of the structure and activity of nerves and neuronal ensembles in vilro, and lay the groundwork for in vivo application as well as providing the preliminary results necessary fbr a successful ROI application. This work not only has potential ^plication as a complement to electrophysiology, but would be of use where optical detection could be siibstituted where introduction of a large number of electrodes can be problematic and in clinically important regions, such as the retina, that are largely inaccessible with current techniques.

这种ROO过渡应用于光学方法的发展，用于成像瞬态结构变化， 神经活动整合了严格的物理学，生物医学和神经科学研究，以进一步实现原始的研究目标。 K99/R00提案。辅导（K99）阶段允许B博士。海尔公园成功转型为独立研究 她在滨江的加州大学生物工程系工作。该职位的参数规定， 启动资金，空间和支持是可比的或超过其他最近聘请的教师。此外，它还提供 至少有75%的研究时间受到保护，并有大量的教学救济。独立（ROO）阶段将进一步博士。 朴的发展作为一个独立的研究人员，并促进成功申请独立研究资助（ROl）。 在完成了物理学研究生研究后，他非常关注生物医学成像，特别是在开发和 在偏振敏感光学相干断层扫描的临床应用中，Park博士补充了这一基础， 生物医学光学与课程作业和动手实验室经验与当前的神经科学技术。他的研究 该提案解决了对神经结构和活动的非接触式检测的需要。当前的动作检测技术 潜在的传播涉及与电极的直接接触或引入化学标记物，例如Ca ^^染料。的焦点 这种K99/R00是使用相位敏感的光学检测器，对伴随神经元中的尖峰传播的瞬时结构变化进行光学检测。 干涉测量技术被称为谱域光学相干断层扫描（SD-OCT）。的重要的第一步 光学检测神经中的活动是在SD-OCT图像中识别它们的能力。在辅导阶段（K99）， 表明无髓鲎外侧复合视神经和有髓大鼠坐骨神经可与周围神经隔离 其他组织类型的结构图像，提供了有价值的基础，为未来在体内应用的光学检测的神经 活性，因为它允许在获取的图像内区分有髓鞘和无髓鞘神经元与其他组织类型。 这些结果还表明，神经结构的光学图像的定量分析是能够无损的 评估神经髓鞘形成的程度。电记录动作电位传播与 建立了相关轴突厚度变化的光学检测，并且这种检测对于单脉冲是可能的，而不需要 需要对重复试验的结果求平均值。本提案的独立阶段旨在进一步发展这种光学 技术，以便它可以应用于详细研究单个细胞之间的相互作用和功能性细胞的行为。 神经网络成功完成拟议的研究将使光学检测的结构和活动的神经 和神经元系综的体外研究，为在体应用奠定基础，并提供初步结果 成功的ROI应用程序所必需的。这项工作不仅具有潜在的应用，作为电生理学的补充， 在引入大量电极可能有问题的情况下 以及在临床上重要的区域，例如视网膜中，这在很大程度上是当前技术无法达到的。