Apoptosis in the Generation of T-cell Memory

研究：这是一项研究计划，旨在研究细胞凋亡在T细胞记忆发展中的作用。 在感染后，T细胞经历快速克隆扩增，导致数量增加和更高的增殖。 抗原特异性T细胞的频率。最初的扩张阶段之后很快就会进入收缩阶段 （克隆收缩），其中超过90%的抗原特异性T细胞被消除。幸存的 抗原特异性T细胞产生记忆池并提供持久的保护以防止再攻击。研究 旨在阐明细胞凋亡特异性塑造细胞质量的机制。 和发育中的抗原特异性T细胞记忆库的功能。具体而言，我们的目标是： 1)确定死亡受体介导的凋亡途径在细胞凋亡的产生和稳态中的作用。 记忆T细胞区 2)检测Bcl-2调节的线粒体依赖性凋亡途径在细胞凋亡中的作用。 抗原特异性T细胞记忆的发展。 3)检查抗原介导的细胞凋亡过程中增殖和凋亡易感性之间的关系。 免疫反应中的T细胞记忆的产生。 这项拟议的研究将我们对T细胞功能的理解与目前关于T细胞作用的想法结合起来。 凋亡^候选人：本辛格博士以优异成绩毕业于宾夕法尼亚大学 兽医学院获得博士学位。2003年，宾夕法尼亚大学 在劳伦斯图尔卡博士的指导下学习医学。他的免疫学论文主要是关于 调节性T细胞的发育和功能。他目前是拉霍亚研究所的研究科学家， 过敏和免疫学（L1 AI）在道格拉斯绿色博士的实验室。* 环境：绿色博士是 目前是LIAI细胞免疫学的负责人，并被公认为两个领域的专家， 免疫学和细胞凋亡，并有杰出的出版记录。他有很好的训练记录 独立科学家LIAI是国际公认的免疫学领域的领导者， 分子细胞生物学，并提供了一个良好的环境与必要的内部设施，以进行 建议的研究。

Research: This is a research proposal to examine the role of apoptosis in the development of T cell memory. Upon infection, T cells undergo rapid clonal expansion resulting in both increased numbers and higher frequency of antigen specific T cells. An initial expansion phase is quickly followed by a contraction phase (clonal contraction) where more than 90% of the antigen specific T cells are eliminated. The surviving antigen specific T cells create the memory pool and provide durable protection from re-challenge. The studies proposed herein are designed to elucidate the mechanisms by which apoptosis specifically shapes the quality and function of the developing antigen specific T cell memory pool. In particular, our aims are to: 1) Determine the role of death receptor mediated apoptosis pathways in the generation and homeostasis of the memory T cellcompartment. 2) Examine the function of the Bcl-2 regulated, mitochondrial dependent apoptosis pathway in the development of the antigen specific T cell memory. 3) Examine the relationship between proliferation and susceptibility to apoptosis during antigen mediated immune responses in the generation of T cell memory. The proposed research integrates our understanding of T cell function with current ideas regarding the role of apoptosis. ^Candidate: Dr. Bensinger graduated summa cum laude from the University of Pennsylvania School of Veterinary Medicine and received his Ph.D. (2003) from University of Pennsylvania School of Medicine under the mentorship of Dr. Laurence Turka. His thesis in immunology was focused on the development and function of regulatory T cells. He is currently a research scientist at the La Jolla Instiute for Allergy and Immunology (L1AI) in the laboratory of Dr. Douglas Green. *Environment: Dr. Green is currently the head of cellular immunology at LIAI, and is recognized as an expert in both the fields of immunology and apoptosis with an outstanding publication record. He has a strong track record of training independent scientists. LIAI is an internationally recognized leader in both the fields of immunology and molecular cell biology and provides an excellent environment with the necessary in house facilities to conduct the proposed research.