Purchase of an X-Ray Generator for Macromolecular Crystallography

购买用于高分子晶体学的 X 射线发生器

• 批准号: 7215097
• 负责人: Steven D Bruner
• 金额: $ 22.8万
• 依托单位: BOSTON COLLEGE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7215097
• 关键词: 20 year old; Aging; Area; Arts; Biochemistry; Biological Factors; Biology; Biomimetics; Boston; Catabolism; Chemicals; Chemistry; Complex; Crystallography; DNA; Enzymes; Funding; Human; Hydrolase; Inositol Metabolism Pathway; Laboratories; Laboratory Research; Maintenance; Minor; Mitochondria; Molecular; Organic Chemistry; Proteins; Range; Research; Research Activity; Research Project Grants; Roentgen Rays; Structure; Students; Transfer RNA; United States National Institutes of Health; X-Ray Crystallography; base; catalyst; college; cryogenics; detector; improved; inhibitor/antagonist; instrument; macromolecule; programs; repaired; structural biology

DESCRIPTION (provided by applicant): The chemistry department at Boston College is seeking funds to purchase a high flux X-ray generator for our macromolecular diffractometer. The instrument will provide a much needed upgrade to our existing setup which functions poorly due to our aging and unreliable generator. The new instrument will provide analytical support for a very broad range of research programs in seven different laboratories, as listed below. These research activities are concentrated primarily in the areas of chemical biology, biochemistry, and synthetic organic chemistry: Major Users: Prof. Steven D. Bruner: "Structural biology of complex natural product biosynthetic enzymes" Prof. Evan Kantrowitz: "The molecular basis of cellular control mechanisms" Prof. Shana O. Kelley: "Human mitochondrial tRNA structure and function" Prof. Larry W. McLaughlin: "Studies of complex DNA assemblies" Prof. Mary F. Roberts: "Enzymes of inositol metabolism and catabolism" Minor users: Prof. Scott J. Miller: "Biomimetic catalysts for asymmetric synthesis" Prof. Marc L. Snapper: "Improved inhibitors of S-adenosylhomocysteine hydrolase" The new instrument will enhance the research of graduate students, undergraduates, and postdocs. Collectively, the above seven research laboratories comprise a group of ~ 62 graduate students, 15 postdocs, and 20 undergraduate research students. X-ray crystallography is frequently the best experimental approach to the structure determination of protein, DNA and small molecular/macromolecule complexes. Access to a reliable and efficient X-ray diffractometer will both facilitate our current research and open new avenues of research. Presently, we have in place all of the components of a state-of-the-art macromolecular diffractometer except for the X-ray generator. Our current machine is approximately 20 years old and is in constant need of repair and maintenance. In addition, the X-ray flux produced by our current setup is not sufficient for the types of problems in our NIH funded research projects. We have in place a new detector and cryogenic apparatus, and the acquisition of a new generator will provide us with a modern and efficient macromolecular diffractometer.

描述（由申请人提供）：波士顿学院化学系正在寻求资金，为我们的大分子衍射仪购买高通量X射线发生器。该仪器将为我们现有的设置提供急需的升级，由于我们的发电机老化和不可靠，现有的设置功能很差。新仪器将为七个不同实验室的广泛研究项目提供分析支持，如下所列。这些研究活动主要集中在化学生物学，生物化学和合成有机化学领域： 主要用户： 教授Steven D.布鲁纳：“复杂天然产物生物合成酶的结构生物学” 教授Evan Kantrowitz：“细胞控制机制的分子基础” 教授莎娜·奥Kelley：“人类线粒体tRNA结构和功能” 教授拉里·W McLaughlin：“复杂DNA组装的研究” 教授玛丽F. Roberts：“肌醇代谢酶和catenin” 次要用户： 教授Scott J.米勒：“用于不对称合成的仿生催化剂” 教授马克湖Snapper：“改良的S-腺苷高半胱氨酸水解酶抑制剂” 新仪器将加强研究生、本科生和博士后的研究。总的来说，上述七个研究实验室包括一组约62名研究生，15名博士后和20名本科研究生。X射线晶体学通常是测定蛋白质、DNA和小分子/大分子复合物结构的最佳实验方法。获得可靠高效的X射线衍射仪将促进我们目前的研究，并开辟新的研究途径。 目前，我们已经拥有了最先进的高分子衍射仪的所有组件，除了X射线发生器。我们目前的机器大约有20年的历史，需要经常维修和保养。此外，我们目前的设置产生的X射线通量不足以解决我们NIH资助的研究项目中的问题。我们已经安装了一个新的探测器和低温设备，购置一个新的发生器将为我们提供一个现代化和高效的大分子衍射仪。