An FT Mass Spectrometer to Identify Posttranslational Modifications

描述（由申请人提供）：这是一份LTQ-Orbitrap质谱仪2D LC蛋白质组学系统的申请。该系统将用于描述调节细胞生长和分裂、转录和其他重要事件的蛋白质的翻译后修饰。蛋白质的翻译后修饰控制着它们的活性和相互作用，以及它们的稳定性和降解。研究的生物系统范围从癌症和逆转录病毒调控到神经元存活和退化。所要求的新开发仪器与以前可用的表征蛋白质修饰的设备相比具有许多优点。傅里叶变换质谱仪为前驱体和碎片离子测量提供了高质量精度（<5 ppm），并结合了高分辨率（高达60,000）。这些特性大大提高了对蛋白质鉴定的信心。该设备在MS2和MS3模式下执行数据依赖扫描的能力对于磷酸化位点的明确表征至关重要。质量分析仪的线性离子阱和傅立叶变换轨道阱的灵敏度允许检测次要成分和亚化学计量修改。对于包含变量修改的复杂数据集的分析，强大的数据分析系统是必不可少的。该设备将极大地加强索尔克研究所7个主要用户和2个次要用户的研究，他们的工作得到了许多NIH拨款的支持。

DESCRIPTION (provided by applicant): This is a request for an LTQ-Orbitrap Mass Spectrometer 2D LC Proteomics System. The System will be used to characterize posttranslational modification in proteins regulating cell growth and division, transcription and other important events. Posttranslational modifications of proteins control their activity and interactions as well as their stability and degradation. The biological systems investigated range from cancer and retroviral regulation to neuronal survival and degeneration. The newly developed instrumentation requested possesses numerous advantages over previously available equipment for the characterization of protein modifications. The Fourier transform mass spectrometer affords high mass accuracy (<5 ppm) for both precursor and fragment ion measurements combined with high resolution (up to 60,000). These properties result in greatly enhanced confidence in protein identification. The equipment's ability to perform data dependent scans in both MS2 and MS3 mode is essential for the unambiguous characterization of phosphorylation sites. The sensitivity of both components of the mass analyzer, the linear ion trap and the Fourier transform Orbitrap, allows the detection of minor components and sub-stoichiometric modifications. The powerful data analysis system is indispensable for the analysis of complex data sets containing variable modifications. The equipment will greatly enhance the research of 7 major and 2 minor users at the Salk Institute whose work is supported by numerous NIH grants.