CORE--PROTEINS AND THEIR POSTTRANSLATIONAL MODIFICATIONS

蛋白质特性对于研究 控制细胞的生长和分化。部分的确定 氨基酸序列通常是表征的关键一步 一个新奇的因素。这个核心将同时使用化学测序和质量 光谱分析以阐明所研究蛋白质的一级结构 通过这个程序。这两种方法的结合确保了最佳 在描述少量新奇事物时的敏感性和准确性 蛋白质。此外，核心将描述翻译后的特点 磷酸化等修饰在细胞周期中起重要作用 多种参与转录的蛋白质活性的调节 监管和信号传递。质谱分析与原子吸收光谱的结合 经典的Edman退化在这一领域特别有利。 核心还将验证重组蛋白的身份用于 这些项目。对所使用的蛋白质试剂的全面表征 实验对于准确解释数据是必不可少的。 此外，核心将保持灵活性，以满足对其提出的新要求 通过将部分资源投入到以下领域的方法开发 蛋白质特性。

Protein Characterization is essential for the study of factors that control cell growth and differentiation. Determination of a partial amino acid sequence is often a crucial step in the characterization of a novel factor. This Core will employ both chemical sequencing and mass spectral analysis to elucidate the primary structure of proteins studied by this program. The combination of these two approaches ensures optimal sensitivity and accuracy when characterizing small quantities of novel proteins. In addition, the Core will characterize post-translational modifications such as phosphorylation which play an important role in the modulation of activity of many proteins involved in transcriptional regulation and signaling. The combination of mass spectral analysis with classical Edman degradation is especially advantageous in this field. The Core will also verify the identity of recombinant proteins used in the projects. A full characterization of the protein reagents used in experiments is essential for accurate interpretation of data. Furthermore, the Core will remain flexible to new demands placed upon it by devoting part of its resources to method development in the field of protein characterization.