CORE--PROTEINS AND THEIR POSTTRANSLATIONAL MODIFICATIONS

蛋白质表征对于研究 控制细胞生长和分化。 确定部分 氨基酸序列通常是表征 一个新的因素。 这个核心将采用化学测序和质量分析 光谱分析以阐明所研究蛋白质的一级结构 通过这个程序。 这两种方法的结合确保了最佳的 当表征少量新的 proteins. 此外，核心将表征翻译后 修饰，如磷酸化，这在细胞凋亡中起着重要作用。 参与转录的许多蛋白质的活性的调节 调节和信号。 质谱分析与 经典的Edman降解在该领域中特别有利。 核心还将验证用于 项目。 用于蛋白质试剂的完整表征 实验对于准确解释数据至关重要。 此外，核心将保持灵活性，以满足对其提出的新要求 通过将其部分资源用于以下领域的方法开发， 蛋白质表征

Protein Characterization is essential for the study of factors that control cell growth and differentiation. Determination of a partial amino acid sequence is often a crucial step in the characterization of a novel factor. This Core will employ both chemical sequencing and mass spectral analysis to elucidate the primary structure of proteins studied by this program. The combination of these two approaches ensures optimal sensitivity and accuracy when characterizing small quantities of novel proteins. In addition, the Core will characterize post-translational modifications such as phosphorylation which play an important role in the modulation of activity of many proteins involved in transcriptional regulation and signaling. The combination of mass spectral analysis with classical Edman degradation is especially advantageous in this field. The Core will also verify the identity of recombinant proteins used in the projects. A full characterization of the protein reagents used in experiments is essential for accurate interpretation of data. Furthermore, the Core will remain flexible to new demands placed upon it by devoting part of its resources to method development in the field of protein characterization.