A State-of-the-art BIACORE for UCSF Mission Bay

摘要/摘要我们正在申请一台BIACORE T100仪器，为UCSF的多名研究人员提供使用表面等离子体共振（SPR）技术定量分析蛋白质-蛋白质和蛋白质-配体相互作用的能力。目前唯一可用的SPR仪器在UCSF扩建的Mission Bay校区是Biacore 1000。实验需求和使用已经增长，因此B1000是不够的。随着加州大学旧金山分校使命湾校区研究设施的快速扩张，有必要确保第二台功能更强大的仪器。Biacore T100提供的灵敏度、温度控制、自动化和易用性的改进，比目前的Biacore 1000有了显著的提高。它将实现实验方法，如高通量采样和结合热力学分析，这在Biacore 1000上是不可能的。在现有Biacore 1000的补充下，T100将简化SPR仪器的培训和使用，以便更多的研究人员熟悉这项技术，并可能计划在他们的工作中使用它。Biacore T100的增加将使加州大学旧金山分校的大量科学家受益，并使研究人员能够充分利用SPR技术的能力。该提案的主要用户来自十个不同的部门，包括UCSF综合癌症中心、定量生化研究所、UCSF糖尿病中心和Gladstone研究所。生物分子结合相互作用的表征是所有这些研究人员研究计划的核心，所有研究人员都将受益于T100的卓越灵敏度和高通量能力。调查人员承诺共享该仪器，并将积极主动地将其提供给其他用户。这将包括为该仪器的新用户或偶尔用户提供获得高技能核心用户服务的机会。T100将极大地促进正在进行的、由美国国立卫生研究院资助的基础研究，这些研究的基本长期目标是理解生物学和医学相关相互作用背后的化学和物理原理。药物的发现取决于对人类疾病机制的了解。开发候选药物的化学合成策略依赖于它们与蛋白质靶标结合的精确测量。本提案中描述的设备是独一无二的，为科学家提供了最敏感的工具，用于检测疾病机制中的生物蛋白相互作用，并用于定量测量结合候选药物与靶蛋白的结合。

DESCRIPTION (provided by applicant): A State-of-the-Art BIACORE for UCSF Mission Bay Project Summary / Abstract We are requesting a Biacore T100 instrument to provide multiple investigators at UCSF the capability of quantifying the analyses of protein-protein and protein-ligand interactions using surface plasmon resonance (SPR) technology. The only currently available SPR instrument at UCSF's expanding Mission Bay campus is a Biacore 1000. Experimental demands and usage have grown so that the B1000 is inadequate. With the fast rate of expansion of research facilities at the UCSF Mission Bay campus, it is necessary to secure a second and more capable instrument. The improved sensitivity, temperature control, automation and ease of use afforded by the Biacore T100 represents a significant enhancement over the current Biacore 1000. It will enable experimental approaches such as high-throughput sampling and analysis of binding thermodynamics that are impossible with the Biacore 1000. Complemented by the current Biacore 1000, the T100 will ease training and the use of SPR instruments so that more researchers will become familiar with the technology and may plan using it in their work. The addition of a Biacore T100 will benefit a large community of scientists at UCSF and allow investigators to exploit the fullest capabilities of SPR technology. The major users on this proposal represent ten different departments and include affiliations with the UCSF Comprehensive Cancer Center, the Institute for Quantitative Biochemical Research, the UCSF Diabetes Center, and the Gladstone Institutes. Characterization of biomolecular binding interactions is central to the research programs of all these investigators, and all will benefit from the exceptional sensitivity and high throughput capacity of the T100. The investigators are committed to sharing the instrument and will be proactive in making it available for other users. This will include providing new or occasional users of the instrument with access to the services of highly skilled core users. The T100 will greatly facilitate ongoing, NIH-funded basic investigations that share the fundamental long-term goal of understanding the chemical and physical principles that underlie biologically and medically relevant interactions. Relevance to Public Health Drug discovery depends on knowing mechanisms of human disease. Developing strategies for the chemical synthesis of candidate drugs depends on accurate measurement of their binding to protein targets. The equipment described in this proposal is unique in providing scientists with the most sensitive tools for detecting biological protein interactions in disease mechanisms and for quantitative measurements of binding drug candidates to target proteins.