A Strain Repository for Dictyostelium discoideum

盘基网柄菌菌株库

• 批准号: 7194090
• 负责人: RICHARD Harry KESSIN
• 金额: $ 20.13万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7194090
• 关键词: Antibodies; Bar Codes; Biological; Biological Availability; Biological Factors; Biological Preservation; Cataloging; Catalogs; Cells; Collection; Communities; Data; Development; Dictyostelium; Dictyostelium discoideum; Documentation; Educational process of instructing; Explosion; Expression Library; Genes; Genomic Library; Grant; Human Resources; Knock-out; Laboratories; Letters; Libraries; Mediating; Messenger RNA; Molecular; Mutagenesis; Numbers; Ontology; Operative Surgical Procedures; Phenotype; Plasmids; Purpose; Quality Control; Reagent; Recovery; Research; Resources; Retirement; Security; Staging; Standardization; Standards of Weights and Measures; Techniques; Work; cDNA Expression; cDNA Library; comparative; homologous recombination; interest; mutant; programs; promoter; repository; research study; response; restriction enzyme

DESCRIPTION (provided by applicant): The current application is for a continuation of The Dictyostelium Stock Center Repository grant. During the initial grant period we have successfully developed an interface with dictyBase, the Dictyostelium central nformational website. We have recovered, annotated, and stored nearly 900 strains and more than 200 useful plasmids. These are in increasing demand by the research community. We provide data and letters from the research and teaching community supporting the utility of this resource. In the current application we propose to increase the collections of strains and plasmids and explain why new research directions demand new material. We propose to complete the recovery, annotation and storage of legacy collections and to incorporate the products of large-scale knockout mutagenesis projects. We will make the collections more useful by collaborating with the personnel of dictyBase to describe the phenotypes of each mutant using the standards developed for Open Biological Ontology. We will continue to recover and annotate plasmids that can be used for research, including integrating and extrachromosomal plasmids and plasmids with a variety of promoters fused to gfp, yfp and other markers. We provide newly made inducible and cre-lox plasmids. These will be provided with as much information as possible. Finally, we will add a new component in the form of other hard to produce reagents including REMI libraries, cDNA expression libraries, and, as they become available, collections of bar-coded mutants. The Stock Center will continue to increase the efficiency of research and to guarantee the security of availability of biological resources.

描述（由申请人提供）：当前申请是为了继续获得盘基网柄菌库存中心储存库的资助。在最初的赠款期间，我们成功地开发了一个与DictyBase的接口，Dictyosteopathy中央信息网站。我们已经回收、注释和储存了近900株菌株和200多个有用的质粒。这些都是在研究界的需求越来越大。我们提供来自研究和教学界的数据和信件，支持这一资源的实用性。在当前的应用中，我们建议增加菌株和质粒的收集，并解释为什么新的研究方向需要新的材料。我们建议完成传统收藏品的恢复、注释和储存，并纳入大规模敲除诱变项目的产物。我们将通过与dictyBase的工作人员合作，使用为开放生物本体开发的标准来描述每个突变体的表型，从而使这些集合更加有用。我们将继续回收和注释可用于研究的质粒，包括整合质粒和染色体外质粒，以及带有多种融合gfp、yfp和其他标记的启动子的质粒。我们提供新的诱导型和cre-lox质粒。将尽可能多地向他们提供信息。最后，我们将以其他难以生产的试剂的形式添加一个新的组件，包括REMI文库、cDNA表达文库，以及条形码突变体的集合。库存中心将继续提高研究效率，并保证生物资源的安全可用性。