CONTROL OF AGGREGATION IN DICTYOSTELIUM BY CAMP

这项研究将继续表征物理结构 和调节几个基因，这些基因的表达被协调调节， 在网骨藻发育过程中cAMP的作用。 第一个基因是 cAMP磷酸二酯酶，我们已经克隆。 这种基因将进一步 并研究了其调节作用。 第二个基因是抑制剂 cAMP磷酸二酯酶，并被cAMP抑制。 抑制剂 糖蛋白已被纯化，氨基末端序列已被 测定 编码这种糖蛋白的基因将由 同样的技术用于cAMP磷酸二酯酶基因。 第三种蛋白质， cAMP蛋白激酶将与另一个 在实验室中，我们将其与前两个基因进行了比较。 cAMP依赖蛋白 激酶调节和催化亚基基因将从cDNA中回收 用cAMP诱导的mRNA制备文库并将其引入λ 矢量gt11。 将分析几种缺乏cAMP磷酸二酯酶的突变体， 确定它们是结构性缺陷还是监管缺陷。 cAMP的作用 依赖蛋白激酶的其他基因的调节将是 分析了 cAMP磷酸二酯酶基因将以一种允许 其表达和用于降解网骨藻和其它细胞中cAMP。 这为研究cAMP代谢提供了新的工具。

The research will continue the characterization of the physical structure and regulation of several genes whose expression is coordinately regulated by cAMP during Dictyostelium development. The first of these genes is the cAMP phosphodiesterase, which we have cloned. This gene will be further characterized and its regulation studied. The second gene is an inhibitor of the cAMP phosphodiesterase and is repressed by cAMP. The inhibitor glycoprotein has been purified and the amino terminal sequence has been determined. The gene coding for this glycoprotein will be cloned by the same techniques used for the cAMP phosphodiesterase gene. A third protein, the cAMP protein kinase will be studied in collaboration with another laboratory and compared to the first two genes. The cAMP dependent protein kinase regulatory and catalytic subunit genes will be recovered from a cDNA library prepared with cAMP induced mRNA and introduced into the lambda vector gt11. Several mutants which lack cAMP phosphodiesterase will be analysed to determine if they are structural or regulatory defects. The role of cAMP dependent protein kinase in the regulation of the other genes will be analysed. The cAMP phosphodiesterase gene will be manipulated in a way which allows its expression and use to degrade cAMP in Dictyostelium and other cells. This should provide a new tool for the study of cAMP metabolism.