Bioassays and Molecular Characterization by Microchip CE

描述(由申请人提供)： 这项研究的目标是开发塑料微芯片分析技术，为对制药和医学研究日益重要的生物和小分子疗法的痕量测定和/或物理性质提供高通量方法。支持这一长期目标的是两个具体目标。 第一个目标是从三个类别开发适当的分析，这三个类别将从微芯片平台的优势中受益匪浅。研究重点将包括与标准免疫分析方法相比，在速度和选择性方面显著改进痕量生物化合物的测定。基于适体的蛋白质分析将被用作说明性的化学系统，而具有激光诱导荧光(LIF)检测的多路塑料芯片上的毛细管电泳将是分析技术。通过快速、高分辨率分离蛋白质-光适配子复合体，分析时间将从几小时缩短到几分钟，而不需要事先准备样品。 此外，将开发一种相对高通量的基于非平衡分离的方法，该方法允许从广泛的配体结合体系中准确地确定Kd和IC-50值。在具有激光诱导荧光、间接激光诱导荧光或流动辅助质谱仪检测的多道塑料芯片上进行的非平衡亲和毛细管电泳将与几种模型配体结合系统一起使用。作为最后的说明，将开发一个通用的、低样品消耗、高通量和低成本的基于分离的平台，以确定小分子(相对分子质量200-1000)的两个关键物理化学性质，即PKA和LOG POW。多道塑料微芯片与间接激光诱导荧光或质谱学检测相结合，可以提供一种快速评估这些关键参数的手段。 第二个目标将是开发适当的塑料微芯片平台，以便进行这些分析。特别是，这些平台必须与第一个特定目标中开发的分析方法兼容。将努力开发具有与激光诱导荧光或间接激光诱导荧光一起使用的适当性能的多路塑料芯片，以及用于激光诱导荧光相关方法的适当激光系统。工作还将侧重于适当的芯片设计和塑料衬底，用于采用质谱学检测方法进行多分析物分析。完成后，将创建适当的微芯片组，用于生物和小分子疗法的分析。

DESCRIPTION (provided by applicant): The goal of this research is to develop plastic microchip analytical technologies that yield high-throughput approaches for the trace determination and/or physical properties of biological and small molecule therapeutics that are increasingly important to pharmaceutical and medical research. In support of this long-term goal are two specific aims. The first aim is to develop appropriate assays from three categories that would benefit significantly from the advantages of the microchip platform. The research focus will include efforts to significantly improve the determination of trace amounts of biological compounds with respect to speed and selectivity as compared with the standard immunoassay methodology. Aptamer-based assays of proteins will be used as illustrative chemical systems, and capillary electrophoresis on a multilane plastic chip with detection by laser induced fluorescence (LIF) will be the analytical technique. Analysis time will be shortened from hours to minutes by rapid, high-resolution separation of protein-photoaptamer complexes without the need for prior sample preparation. Additionally, a relatively high-throughput non-equilibrium separation-based approach that allows the accurate determination of Kd and IC-50 values from a wide range of ligand-binding systems will be developed. Non-equilibrium affinity capillary electrophoresis performed on a multilane plastic microchip with LIF, indirect LIF or flow-assisted mass spectrometry detection will be used with several model ligand-binding systems. As the final illustration, a universal, low sample consumption, high throughput and low cost separation-based platform to determine two of the key physiochemical properties of small molecules (MW 200-1,000), pKa and log Pow will be developed. Multilane plastic microchips coupled with either indirect laser-induced fluorescence or mass spectrometric detection can provide a rapid means of assessing these key parameters. The second aim will be to develop appropriate plastic microchip platforms from which these assays can be performed. In particular, these platforms must be compatible with the assays developed in the first Specific Aim. Efforts will be directed at developing multilane plastic chips having appropriate properties for use with LIF or indirect LIF as well as the appropriate laser system for LIF-associated methods. Efforts will also focus on appropriate chip designs and plastic substrates for multi-analyte analyses with mass spectrometric detection methods. Upon completion, appropriate microchip-sets will be created for assays of biological and small molecule therapeutics.