Acquisition of an LC Mass Spectrometer for Nucleic Acids Research

购买用于核酸研究的 LC 质谱仪

• 批准号: 7795317
• 负责人: PATRICK A LIMBACH
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2011-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795317
• 关键词: Accounting; Address; Antibiotic Resistance; Antineoplastic Agents; Base Sequence; Detection; Digestion; Doctor of Philosophy; Ensure; Equipment; Funding; Future; Goals; High Pressure Liquid Chromatography; Housing; Human Resources; Institution; Maps; Mass Spectrum Analysis; Measurement; Molecular Weight; Nucleic Acids; Nucleic acid sequencing; Nucleosides; Oligonucleotides; Peptides; Pseudouridine; Recovery; Request for Proposals; Research; Research Personnel; Resources; Scanning; Schedule; Scientist; Services; Side; System; Transfer RNA; Universities; Work; cost; crosslink; drug development; instrument; instrumentation; liquid chromatography mass spectrometry; mass spectrometer; nanobiotechnology; programs; public health relevance; square foot

DESCRIPTION (provided by applicant): This proposal requests funds to acquire an ultra-high-performance liquid chromatography mass spectrometry (UPLC-MS) system intended for the identification and structural characterization of nucleic acids, including those containing modified nucleosides. The NIH-funded researchers participating in this request require LC-MS/MS or LC-IMS-MS/MS capabilities to accomplish goals associated with their funded projects. Specifically, the following types of analyses will be enabled by this acquisition: a) Accurate molecular weight measurements of intact nucleic acids; b) Accurate enzymatic mapping of isolated nucleic acids or mixtures of nucleic acids; c) Sequencing of oligonucleotides and enzymatic digestion mixtures by MS/MS; d) Identification of modified nucleosides and their placement within nucleic acid sequences; e) Sequence placement of pseudouridine, the most abundant modified nucleoside, using MRM scans; f) High sensitivity detection and identification of oligonucleotide synthetic side- and by-products; g) Detection of oligonucleotide:peptide cross-links; and h) de novo sequencing of modified RNAs, such as transfer RNAs (tRNAs). The PI, who has been active in the field of mass spectrometry for nearly 20 years, will be responsible for the managerial oversight of the requested instrumentation. He will coordinate oversight with an advisory board composed of scientists from various participating institutions. Together they will establish scheduling priorities, work with accounting personnel to establish cost-recovery rates, and will serve as a resource for projecting future use of the requested equipment. The equipment will be housed in the University of Cincinnati Mass Spectrometry Facility. This 3,500 sq. ft. facility was renovated in 2001 and is equipped with the ancillary instrumentation required for providing the proposed analytical services. The facility's co-directors are two Ph.D.-level staff scientists. One of these scientists will be designated as the primary operator of the new instrument, and it is anticipated that the facility will add a B.S.-level staff technician to ensure that existing and new analytical services are provided in a timely manner. PUBLIC HEALTH RELEVANCE: The UPLC-MS requested in this application will allow NIH-supported researchers access to modern instrumentation necessary for their research programs. These researchers are addressing a variety of health-related issues including: antibiotic resistance, cancer, drug development and nanobiotechnology.

描述（由申请人提供）：本申请申请资金用于购买一套超高效液相色谱质谱（UPLC-MS）系统，用于核酸的鉴定和结构表征，包括那些含有修饰核苷的核酸。参与此请求的nih资助研究人员需要LC-MS/MS或LC-IMS-MS/MS功能来完成与其资助项目相关的目标。具体来说，以下类型的分析将通过此次收购实现：a)完整核酸的精确分子量测量；b)分离的核酸或核酸混合物的准确酶制图；c)寡核苷酸和酶切混合物的MS/MS测序；d)鉴定修饰的核苷及其在核酸序列中的位置；e)伪尿嘧啶（最丰富的修饰核苷）的序列放置，使用MRM扫描；f)寡核苷酸合成副产物的高灵敏度检测和鉴定；g)寡核苷酸检测：多肽交联；h)重新测序修饰的rna，如转移rna （tRNAs）。PI已经在质谱领域活跃了近20年，将负责所要求仪器的管理监督。他将与一个由各参与机构的科学家组成的顾问委员会协调监督工作。他们将共同确定排期优先次序，与会计人员合作确定成本回收率，并将作为预测所要求设备未来使用情况的资源。该设备将被安置在辛辛那提大学的质谱分析设施中。这3500平方英尺。该设施于2001年进行了翻新，并配备了提供拟议分析服务所需的辅助仪器。该设施的联合主管是两位博士级别的科学家。其中一名科学家将被指定为新仪器的主要操作人员，预计该设施将增加一名理学学士级别的工作人员，以确保及时提供现有和新的分析服务。