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Hybrid Transform Mass Spectrometer for Proteomics

用于蛋白质组学的混合变换质谱仪

基本信息

批准号：
6803692
负责人：
PATRICK A LIMBACH
金额：
$ 91.9万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-25 至 2006-04-24
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6803692
关键词：
biomedical equipment purchase biotechnology mass spectrometry proteomics

项目摘要

DESCRIPTION (provided by applicant): This proposal requests support for the acquisition of an ion trap-Fourier transform ion cyclotron resonance hybrid mass spectrometer. This new instrumentation is intended for the identification and quantitation of proteins from protein complexes, whole proteomes and single cells. This instrument is arguably at the forefront of mass spectrometry instrumentation, and represents a new platform for undertaking sophisticated proteomics-related studies. Proposed use of this instrumentation will support and enhance existing NIH-funded projects and provide avenues of research support that can lead to new funding for future projects. Four NIH-supported users of this equipment will form the core research team and the value added to their research activities by this instrumentation is discussed. All of these individuals currently interact with the University of Cincinnati Mass Spectrometry (UC MS) Facility, and the identified projects are untenable or pain-staking using the equipment already in place in the UC MS Facility. Their research interests, in some form or another, cover a wide spectrum of proteomics-related investigations. Examples include the effects of environmental agents such as estrogens, metals and PAH's on proteomes; examining the underlying biochemistry associated with microorganisms as they relate to biofilm production; and understanding the lamellar body proteome. All of the investigators participating in this instrument request have vigorous and well-funded research programs. In addition, a number of other investigator's NIH-funded research would benefit from this instrument acquisition. The PI, who has been active in the field of mass spectrometry for nearly 15 years, will be responsible for the managerial oversight of the requested instrumentation. He will report to an internal advisory board composed of scientists from various UC campuses and affiliates. The internal advisory board will establish scheduling priorities, work with the PI and accounting personnel to establish cost-recovery rates, and will serve as a resource for projecting future use of the requested equipment. The equipment will be housed in the University of Cincinnati Mass Spectrometry Facility. This 3,500 sq. ft. facility was renovated in 2001 and is equipped with the ancillary instrumentation required for proteomics. The instrumentation will be operated and maintained by two Ph.D.-level facility staff scientists, with one of these scientists having prior experience in both FTMS and proteomics being designated as the primary operator. These staff scientists are supported by B.S.-level staff technicians to ensure that the services are provided in a timely manner.

描述（由申请人提供）：该提案要求支持购置离子阱-傅立叶变换离子回旋共振混合质谱仪。这种新仪器用于从蛋白质复合物、全蛋白质组和单细胞中鉴定和定量蛋白质。该仪器可以说处于质谱仪器的最前沿，代表了进行复杂蛋白质组学相关研究的新平台。该仪器的拟议使用将支持和加强现有的NIH资助的项目，并提供研究支持的途径，可以为未来的项目带来新的资金。该设备的四个NIH支持的用户将形成核心研究团队，并讨论了这种仪器对他们的研究活动的附加值。所有这些人目前都与辛辛那提大学质谱（UC MS）设施进行互动，并且所确定的项目无法使用UC MS设施中已经到位的设备。他们的研究兴趣，以某种形式或另一种，涵盖了广泛的蛋白质组学相关的调查。例子包括环境因素如雌激素，金属和多环芳烃对蛋白质组的影响;检查与微生物相关的生物化学，因为它们与生物膜生产有关;并了解板层体蛋白质组。所有参与这项仪器要求的研究人员都有积极和资金充足的研究计划。此外，一些其他研究人员的NIH资助的研究将受益于这一仪器的收购。 PI在质谱领域活跃了近15年，将负责所需仪器的管理监督。他将向一个由来自加州大学各个校区和附属机构的科学家组成的内部顾问委员会报告。内部咨询委员会将确定日程安排的优先次序，与PI和会计人员合作确定成本回收率，并将作为预测所需设备未来使用情况的资源。设备将存放在辛辛那提大学质谱设施中。这3,500平方米。英尺该设施于2001年进行了翻新，并配备了蛋白质组学所需的辅助仪器。仪器将由两名博士操作和维护-一名高级设施工作人员科学家，其中一名在FTMS和蛋白质组学方面具有经验的科学家被指定为主要操作员。这些科学家由B.S.-一级技术人员，以确保及时提供服务。