Acquisition of a High Resolution Mass Spectrometer for the University of Cincinna

描述(由申请人提供)：该提案要求获得资金，以获得高分辨率傅立叶变换质谱仪和相关的液相色谱系统，以支持美国国立卫生研究院资助的辛辛那提大学研究人员的研究。这一新仪器旨在支持由美国国立卫生研究院资助的正在进行的与健康相关问题的研究，如抗生素耐药性、动脉粥样硬化、癌症、糖尿病、传染病和肥胖症。具体地说，此次收购将使以下类型的分析成为可能：a)新陈代谢流感测量，以确定心脏代谢性疾病和各种癌症的关键代谢途径；b)代谢物鉴定，以支持在新陈代谢流量测量期间揭示的代谢途径的鉴定；以及c)溶血磷脂和胆固醇代谢途径的代谢组特征。辛辛那提大学由美国国立卫生研究院支持的一批关键研究人员将直接受益于这些新能力。此外，这些新的能力将刺激这些研究人员和校园里的其他生物医学研究人员检查将从该仪器中受益的额外实验。参与这项申请的NIH资助的研究人员需要专用的LC-MS/MS或高分辨率ESI/APCI-MS/MS能力来实现与其资助项目相关的目标。PI在质谱学领域已经活跃了20多年，他将负责对所要求的仪器进行管理监督。他将向外部顾问委员会汇报工作，该委员会由大学内部的科学家组成。咨询委员会将确定时间表的优先次序，与私人投资和会计人员合作，确定费用回收率，并将作为预测所需设备未来使用情况的资源。这些设备将安置在辛辛那提大学的质谱学设施中。这个3500平方英尺。英国《金融时报》设备配备了生物分子表征所需的辅助仪器。该仪器将由两名博士级别的设施工作人员科学家操作和维护，其中一名科学家被指定为主要操作员。

DESCRIPTION (provided by applicant): This proposal requests funds to acquire a high resolution Fourier transform mass spectrometer and associated liquid chromatography system to support the NIH-funded research of investigators at the University of Cincinnati. This new instrumentation is intended to support on- going NIH-funded research into health-related issues such as antibiotic resistance, atherosclerosis, cancer, diabetes, infectious diseases and obesity. Specifically, the following types of analyses will be enabled by this acquisition: a) Metabolic flu measurements to identify key metabolic pathways in cardiometabolic diseases and various cancers; b) Metabolite identification to support identification of metabolic pathways revealed during metabolic flux measurements; and c) metabolomic characterization of lysophospholipid and cholesterol metabolic pathways. A key group of NIH-supported investigators at the University of Cincinnati will directly benefit from these new capabilities. Moreover, these new capabilities will stimulate these researchers and other biomedical investigators on campus to examine additional experiments that will benefit from this instrument. The NIH-funded researchers participating in this request require dedicated LC-MS/MS or high-resolution ESI/APCI-MS/MS capabilities to accomplish goals associated with their funded projects. The PI, who has been active in the field of mass spectrometry for over 20 years, will be responsible for the managerial oversight of the requested instrumentation. He will report to an external advisory board composed of scientists from within the university. The advisory board will establish scheduling priorities, work with the PI and accounting personnel to establish cost-recovery rates, and will serve as a resource for projecting future use of the requested equipment. The equipment will be housed in the University of Cincinnati Mass Spectrometry Facility. This 3,500 sq. ft. facility is equipped with the ancillary instrumentation required for biomolecule characterization. The instrumentation will be operated and maintained by two Ph.D.-level facility staff scientists, with one of these scientists being designated as the primary operator.